ArticleJournal of biomolecular techniques : JBT2021
Factors influencing recovery of SARS-CoV-2 RNA in raw sewage and wastewater sludge using polyethylene glycol-based concentration method.
Article in Journal of biomolecular techniques : JBT, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.
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Who cites it
7 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.
- Detecting SARS-CoV-2 in sludge samples: A systematic review.The Science of the total environment · 2023Pooled it
- A scoping review of global SARS-CoV-2 wastewater-based epidemiology in light of COVID-19 pandemic.Heliyon · 2024Article
- Monkeypox viral nucleic acids detected using both DNA and RNA extraction workflows.The Science of the total environment · 2023Article
- Comparison of NanotrapFrontiers in microbiology · 2023Article
- Comparison of Electronegative Filtration to Magnetic Bead-Based Concentration and V2G-qPCR to RT-qPCR for Quantifying Viral SARS-CoV-2 RNA from Wastewater.ACS ES&T water · 2022Article
- Making waves: Wastewater surveillance of SARS-CoV-2 in an endemic future.Water research · 2022Article
- Development of an efficient wastewater testing protocol for high-throughput country-wide SARS-CoV-2 monitoring.The Science of the total environment · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wastewater surveillance for monitoring severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important epidemiologic tool for the assessment of population-wide coronavirus disease 2019 (COVID-19). This tool can be successfully implemented only if SARS-CoV-2 RNA in wastewater can be accurately recovered and quantified. The lack of standardized procedure for wastewater virus analysis has resulted in varying SARS-CoV-2 concentrations for the same sample. This study reports the effect of 4 key factors-sample volume, percentage polyethylene glycol (PEG)-NaCl, incubation period, and storage duration at 4°C-on the recovery of spiked noninfectious SARS-CoV-2 RNA in raw sewage and sludge samples. N1 and N2 genes of SARS-CoV-2 were quantified using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and digital droplet PCR (RT-ddPCR) techniques. Results indicate that
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Registered trials
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