ArticleNature methods2022
Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 102 citations in OpenAlex.
- Years-long stability of X10 expansion microscopy hydrogels.Journal of biological engineering · 2026Article
- Imaging of biofilms using cryo-scanning electron microscopy.Essays in biochemistry · 2026Review
- An adaptable, self-organizing, single-cell morphology circuit optimizes suctorian predatory trap structure.Current biology : CB · 2026Article
- Super-resolution expansion microscopy reveals nanoscale protein domains and CObioRxiv : the preprint server for biology · 2026Article
- IntAct-U-ExM enables super-resolution imaging of isoform-specific actin networks across species.PLoS biology · 2026Article
- HAK-actin, a U-ExM-compatible probe to image the actin cytoskeleton.Cell reports methods · 2026Article
- Article
- Axonemal Dynein Visualized in Primary Cilia via Expansion Microscopy.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Landscape expansion microscopy reveals interactions between membrane and phase-separated organelles.The Journal of cell biology · 2026Article
- Septin-mediated coupling of protein import and division during chloroplast evolution.bioRxiv : the preprint server for biology · 2026Article
- An Advanced Mobile Laboratory to enable field-based microbial ecology and cell biology across scales.bioRxiv : the preprint server for biology · 2026Article
- Microscopy in mitochondrial research - a comprehensive review.Journal of cell science · 2026Review
- Functional recovery of the adult murine hippocampus after cryopreservation by vitrification.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Dual-Color Expansion Microscopy of Membrane Proteins Using Bioorthogonal Labeling.Nano letters · 2026Article
- Ultrastructural expansion microscopy reveals unexpected levels of glycosome heterogeneity in African trypanosomes.Journal of microscopy · 2026Article
- Imaging malaria parasites across scales and time.Journal of microscopy · 2026Review
- A centrally positioned cluster of multiple centrioles in antigen-presenting cells fosters T cell activation.Nature communications · 2026Article
- Visualising viral interactions and mechanisms at the nanoscale with expansion microscopy.Npj viruses · 2026Review
- Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy.bioRxiv : the preprint server for biology · 2026Article
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4 authors at 1 institution in 1 country.
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Abstract
Cryofixation has proven to be the gold standard for efficient preservation of native cell ultrastructure compared to chemical fixation, but this approach is not widely used in fluorescence microscopy owing to implementation challenges. Here, we develop Cryo-ExM, a method that preserves native cellular organization by coupling cryofixation with expansion microscopy. This method bypasses artifacts associated with chemical fixation and its simplicity will contribute to its widespread use in super-resolution microscopy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.