ArticleCommunications biology2022
Effective prediction of biosynthetic pathway genes involved in bioactive polyphyllins in Paris polyphylla.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 0 citations in OpenAlex.
- Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plantSynthetic and systems biotechnology · 2026Article
- The potential of metabolic engineering for sustainable phytosterol production.Advanced biotechnology · 2026Review
- Plastid 2-oxoglutarate-dependent dioxygenases mediate stereoselective C14β-hydroxylation in cardenolide biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Transcriptome Analysis and Functional Validation of JAZ Subfamily Genes ofInternational journal of molecular sciences · 2026Article
- Multi-dimensional identification and quality evaluation ofFrontiers in plant science · 2026Article
- Reconstruction ofEngineering in life sciences · 2026Article
- A review of glycosylation diversity and biosynthetic advances of steroidal saponins inFrontiers in plant science · 2026Review
- Multi-omics and field experiments reveal the mechanism of Pseudomonas palleroniana P6 promoting the growth and polyphyllins accumulation in Paris polyphylla.BMC plant biology · 2025Article
- Transcriptome and metabolome reveal the accumulation of secondary metabolites in different species of Dioscorea.BMC plant biology · 2025Article
- The biosynthesis of trillin 6'-Synthetic and systems biotechnology · 2025Article
- The genusHorticulture research · 2025Article
- Dissection of transcriptome and metabolome insights into the polyphyllin biosynthesis in Paris.BMC plant biology · 2025Article
- Metabolomics and transcriptomics profiling of three Paris species with varied polyphyllin compositions.Scientific data · 2024Article
- Genomic insights into Aspergillus tamarii TPD11: enhancing polyphyllin production and uncovering potential therapeutic applications.BMC genomics · 2024Article
- Trans2express - de novo transcriptome assembly pipeline optimized for gene expression analysis.Plant methods · 2024Article
- Study on Synthesis and Regulation of PPVI and PPVII inMetabolites · 2024Article
- Genome-Wide Comparative Profiles of Triterpenoid Biosynthesis Genes in Ginseng and Pseudo Ginseng Medicinal Plants.Life (Basel, Switzerland) · 2023Article
- A novel sterol glycosyltransferase catalyses steroidal sapogenin 3-O glucosylation from Paris polyphylla var. yunnanensis.Molecular biology reports · 2023Article
- Illuminating the biosynthesis pathway genes involved in bioactive specific monoterpene glycosides in Paeonia veitchii Lynch by a combination of sequencing platforms.BMC genomics · 2023Article
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9 authors at 5 institutions in 1 country.
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Abstract
The genes in polyphyllins pathway mixed with other steroid biosynthetic genes form an extremely complex biosynthetic network in Paris polyphylla with a giant genome. The lack of genomic data and tissue specificity causes the study of the biosynthetic pathway notably difficult. Here, we report an effective method for the prediction of key genes of polyphyllin biosynthesis. Full-length transcriptome from eight different organs via hybrid sequencing of next generation sequencingand third generation sequencing platforms annotated two 2,3-oxidosqualene cyclases (OSCs), 216 cytochrome P450s (CYPs), and 199 UDP glycosyltransferases (UGTs). Combining metabolic differences, gene-weighted co-expression network analysis, and phylogenetic trees, the candidate ranges of OSC, CYP, and UGT genes were further narrowed down to 2, 15, and 24, respectively. Beside the three previously characterized CYPs, we identified the OSC involved in the synthesis of cycloartenol and the UGT (PpUGT73CR1) at the C-3 position of diosgenin and pennogenin in P. polyphylla. This study provides an idea for the investigation of gene cluster deficiency biosynthesis pathways in medicinal plants.
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