ArticleCommunications biology2022
Demonstration of targeted crossovers in hybrid maize using CRISPR technology.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Do maize chromosomes contribute to genetic variance in proportion to their size in elite populations?The plant genome · 2026Article
- The Potential of NGTs to Overcome Constraints in Plant Breeding and Their Regulatory Implications.International journal of molecular sciences · 2025Review
- Spatio-temporal regulation of recombinase expression enables efficient autoexcision of selectable marker genes in soybean and maize.BMC plant biology · 2025Article
- CRISPR/Cas9-induced breaks are insufficient to break linkage drag surrounding the ToMV locus of Solanum lycopersicum.G3 (Bethesda, Md.) · 2025Article
- The future of genome editing in plants.Nature plants · 2025Review
- Contribution of crossing over to genetic variance in maize and wheat populations.The plant genome · 2025Article
- dCas9-SPO11-1 locally stimulates meiotic recombination in rice.Frontiers in plant science · 2025Article
- Computationally derived RNA polymerase III promoters enable maize genome editing.Frontiers in plant science · 2025Article
- Impact of predictive selection of LbCas12a CRISPR RNAs upon on- and off-target editing rates in soybean.Plant direct · 2024Article
- Whole-genome versus per-chromosome targeted recombination: Simulations and predicted gains in maize with an integer programming model.The plant genome · 2023Article
- Comprehensive dissection of meiotic DNA double-strand breaks and crossovers in cucumber.Plant physiology · 2023Article
- Efficient targeted recombination with CRISPR/Cas9 in hybrids of Caenorhabditis nematodes with suppressed recombination.BMC biology · 2023Article
- Exploring impact of recombination landscapes on breeding outcomes.Proceedings of the National Academy of Sciences of the United States of America · 2023Review
- Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis.Planta · 2023Review
- Current and Prospective Applications of CRISPR-Cas12a in Pluricellular Organisms.Molecular biotechnology · 2023Review
- Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis.G3 (Bethesda, Md.) · 2022Article
- Nonhomologous end joining as key to CRISPR/Cas-mediated plant chromosome engineering.Plant physiology · 2022Article
- Review
- CRISPR-Cas technology opens a new era for the creation of novel maize germplasms.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Naturally occurring chromosomal crossovers (CO) during meiosis are a key driver of genetic diversity. The ability to target CO at specific allelic loci in hybrid plants would provide an advantage to the plant breeding process by facilitating trait introgression, and potentially increasing the rate of genetic gain. We present the first demonstration of targeted CO in hybrid maize utilizing the CRISPR Cas12a system. Our experiments showed that stable and heritable targeted CO can be produced in F1 somatic cells using Cas12a at a significantly higher rate than the natural CO in the same interval. Molecular characterization of the recombinant plants demonstrated that the targeted CO were driven by the non-homologous end joining (NHEJ) or HDR repair pathways, presumably during the mitotic cell cycle. These results are a step towards the use of RNA-guided nuclease technology to simplify the creation of targeted genome combinations in progeny and accelerate breeding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.