ArticleScientific reports2022
TcTI, a Kunitz-type trypsin inhibitor from cocoa associated with defense against pathogens.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches' Broom Disease: A Systematic Review.International journal of molecular sciences · 2023Pooled it
- Article
- A multiplant transcriptomic atlas reveals conserved and lineage-specific defense architectures in response toProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Peptides Derived From Reactive Center Loops Inhibit Digestive Trypsin-Like Enzymes in Lepidopteran Pests.Archives of insect biochemistry and physiology · 2026Article
- Multi-Transcripts and Expressions ofPlants (Basel, Switzerland) · 2025Article
- Integrative analysis of seed morphology, geographic origin, and genetic structure in Medicago with implications for breeding and conservation.BMC plant biology · 2025Article
- A Novel Kunitz Trypsin Inhibitor fromMolecules (Basel, Switzerland) · 2024Article
- Structural, Biochemical Characterization and Molecular Mechanism of Cerastokunin: A New Kunitz-Type Peptide with Potential Inhibition of Thrombin, Factor Xa and Platelets.The protein journal · 2024Article
- TcSERPIN, an inhibitor that interacts with cocoa defense proteins and has biotechnological potential against human pathogens.Frontiers in plant science · 2024Article
- Enzymatic properties and inhibition tolerance analysis of key enzymes in β-phenylethanol anabolic pathway ofSynthetic and systems biotechnology · 2023Article
- Genetic manipulation of anti-nutritional factors in major crops for a sustainable diet in future.Frontiers in plant science · 2022Review
- Pleiotropic effects of recombinant protease inhibitors in plants.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Protease inhibitors (PIs) are important biotechnological tools of interest in agriculture. Usually they are the first proteins to be activated in plant-induced resistance against pathogens. Therefore, the aim of this study was to characterize a Theobroma cacao trypsin inhibitor called TcTI. The ORF has 740 bp encoding a protein with 219 amino acids, molecular weight of approximately 23 kDa. rTcTI was expressed in the soluble fraction of Escherichia coli strain Rosetta [DE3]. The purified His-Tag rTcTI showed inhibitory activity against commercial porcine trypsin. The kinetic model demonstrated that rTcTI is a competitive inhibitor, with a Ki value of 4.08 × 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.