Evidence map›Paper›PMID 35027456›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2022

Floxed exon (Flexon): A flexibly positioned stop cassette for recombinase-mediated conditional gene expression.

Justin M Shaffer, Iva Greenwald

Open access · hybridAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
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  8. A neurotransmitter atlas ofbioRxiv : the preprint server for biology · 2024
    Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Justin M ShafferDepartment of Biological Sciences, Columbia University, New York, NY 10027.
Iva GreenwaldDepartment of Biological Sciences, Columbia University, New York, NY 10027 isg4@columbia.edu.
Columbia University · US

Funding

Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Aric L Daul, Ann E. Rougvie · 2012 to 2026
$7.5M
CELLULAR AND MOLECULAR FOUNDATIONS OF BIOMEDICAL SCIENCET32GM008798 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI PRYWES, RON M · 2001 to 2018
$6.3M
Regulatory Circuitry and Mechanisms Controlling Cell Fate in C. elegansR35GM131746 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Iva S Greenwald · 2019 to 2026
$5.0M
Developing a novel, rapid-response biosensor and analyzing Notch signaling dynamics in cell fate decisions in vivoF31EY030331 · NEI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI SHAFFER, JUSTIN MATTHEW · 2020 to 2021
$92k
NEI NIH HHS F31 EY030331NIGMS NIH HHS R35 GM131746NIGMS NIH HHS T32 GM008798NIH HHS P40 OD010440
6 · The paper itself

Abstract

Conditional gene expression is a powerful tool for genetic analysis of biological phenomena. In the widely used "lox-stop-lox" approach, insertion of a stop cassette consisting of a series of stop codons and polyadenylation signals flanked by

Indexed as

ExonsGene ExpressionAnimalsCaenorhabditis elegansDNA NucleotidyltransferasesGreen Fluorescent ProteinsIntegrasesPromoter Regions, GeneticRecombinasesRecombination, GeneticRNA InterferenceTransgenesCre recombinaseDNA NucleotidyltransferasesGreen Fluorescent ProteinsIntegrasesRecombinasesSite-specific recombinaseC. elegansconditional gene expressionlox-stop-loxstop cassettetissue-specific RNAi

Identifiers

PMID35027456
PMCPMC8784106
OpenAlexW4205631203

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.