Evidence map›Paper›PMID 35025866›Full record

ArticlePloS one

Calmodulin binds the N-terminus of the functional amyloid Orb2A inhibiting fibril formation.

Maria A Soria, Silvia A Cervantes, Ansgar B Siemer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Role of CPEBs in Learning and Memory.Journal of neurochemistry · 2025
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Maria A SoriaDepartment of Physiology and Neuroscience, Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
Silvia A CervantesDepartment of Physiology and Neuroscience, Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
Ansgar B SiemerDepartment of Physiology and Neuroscience, Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.ORCID https://orcid.org/0000-0003-0901-011X

Funding

Orb2 a functional amyloid in long-term memory: Its structure and how it formsR01GM110521 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI SIEMER, ANSGAR B · 2015 to 2019
$1.9M
NIGMS NIH HHS R01 GM110521
6 · The paper itself

Abstract

The cytoplasmic polyadenylation element-binding protein Orb2 is a key regulator of long-term memory (LTM) in Drosophila. The N-terminus of the Orb2 isoform A is required for LTM and forms cross-β fibrils on its own. However, this N-terminus is not part of the core found in ex vivo fibrils. We previously showed that besides forming cross-β fibrils, the N-terminus of Orb2A binds anionic lipid membranes as an amphipathic helix. Here, we show that the Orb2A N-terminus can similarly interact with calcium activated calmodulin (CaM) and that this interaction prevents fibril formation. Because CaM is a known regulator of LTM, this interaction could potentially explain the regulatory role of Orb2A in LTM.

Indexed as

Amino Acid SequenceAmyloidAnimalsBinding SitesCalmodulinDrosophilaDrosophila ProteinsmRNA Cleavage and Polyadenylation FactorsProtein BindingProtein DomainsProtein IsoformsTranscription FactorsAmyloidCalmodulinDrosophila ProteinsmRNA Cleavage and Polyadenylation FactorsOrb2 protein, DrosophilaProtein IsoformsTranscription Factors

Identifiers

PMID35025866
PMCPMC8758002

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.