ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2022
Fast antibiotic susceptibility testing of urine microflora using a microbiological analyzer based on coherent fluctuation nephelometry.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Deep Learning-Based Rapid Identification ofRisk management and healthcare policy · 2026Article
- A Highly Sensitive Silicon-Core Quantum Dot Fluorescent Probe for Vomitoxin Detection in Cereals.Foods (Basel, Switzerland) · 2025Article
- Low-Cost Electronic Nose for the Determination of Urinary Infections.Sensors (Basel, Switzerland) · 2023Article
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Authors and funding
7 authors.
Funding
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Abstract
backgroundTraditional culture-based microbiological methods remain the most used for defining the etiology of urinary tract infections and antibiotic susceptibility testing (AST) of isolated uropathogens. They are time-consuming and lead to delays of several days when obtaining the final results of microbiological tests.
objectivesIn this study, we validate the possibility of using a microbiological CFN analyzer combined with MALDI-TOF mass spectrometry (MS) for fast conclusive urine testing (1 day) without obtaining pure cultures. MATERIALS AND
methodsThe study included three stages: detection of urine microflora growth using the CFN analyzer to separate positive and negative samples within 2-4 h; fast MS identification of positive samples without isolating uropathogens; fast AST using CFN analyzer within 3-6 h. In parallel, all urine samples were tested by traditional culture-based microbiological methods.
resultIn total, 194 urine samples were tested, and 22 urine cultures were identified by MS, among them, 20 monocultures with bacterial counts ≥ 10
conclusionsThe CFN analyzer is promising and effective for fast AST. Combined with MS identification, it allows to perform full urine analysis in 1 day without the lengthy isolation of pure cultures.
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