Evidence map›Paper›PMID 35025033›Full record

ArticleMolecular biology reports2022

Genome wide analysis of DWARF27 genes in soybean and functional characterization of GmD27c reveals eminent role of strigolactones in rhizobia interaction and nodulation in Glycine max.

Naveed Ur Rehman, Farhat Abbas, Muhammad Imran, Intikhab Alam, Muhammad Imran, Ihteram Ullah, Muhammad Riaz, Fahim Ullah Khan

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Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 20% of its field
1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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4 citing papers in PubMed, 6 citations in OpenAlex.

  1. ThePlants (Basel, Switzerland) · 2026
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4 · The record

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5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Naveed Ur RehmanState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Farhat AbbasThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Muhammad ImranState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Intikhab AlamState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Muhammad ImranCollege of Resources and Environment, Huazhong Agricultural University, Wuhan, China.
Ihteram UllahDepartment of Plant Breeding & Genetics, Gomal University, Dera Ismail Khan, Pakistan.
Muhammad RiazState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresource, Root Biology Center, College of Natural Resource and Environment, South China Agricultural University, Guangzhou, China.
Fahim Ullah KhanDepartment of Agriculture, Hazara University, Mansehra, Pakistan. fahimbiotech@gmail.com.
South China Agricultural University · CNGomal University · PKHuazhong Agricultural University · CN

Funding

International Foundation for Science, Sweden I-1-C-6481-1
6 · The paper itself

Abstract

backgroundStrigolactones (SLs) are newly identified hormones and their biosynthesis is stimulated under phosphate deprivation and accomplished by the action of several enzymes, including the beta-carotene isomerase DWARF27 (D27). Expression of D27 is well renowned to respond to phosphate insufficiency. However, the identification and functional analysis of the carotenoid isomerase D27 genes are not elucidated in soybean. METHODS AND

resultsA total of six D27 genes were identified in the soybean genome and designated on the basis of chromosomal localization. According to the findings, these genes were irregularly distributed on chromosomes, and segmental repetition led to the expansion of the soybean GmD27 gene family. Based on a neighbor-joining phylogenetic tree, the predicted D27 proteins of soybean were divided into three clades. Based on RNA seq data analysis, GmD27 genes were differently expressed in various tissues but GmD27c was the highest. Therefore, GmD27c was chosen for the additional functional study due to its rather obvious transcription in nodulation and roots. RT-qPCR results showed that GmD27c was highly expressed in different nodule stages and in response to rhizobia infection. Functional characterization of GmD27c revealed that overexpression of GmD27c led to higher nodule number, while GmD27c knockdown caused fewer nodules compared to GUS control. Furthermore, GmD27c overexpressed and knockdown lines oppositely regulated the expression of numerous nodulation genes, which are vital for the development of nodules.

conclusionThis study not only discovered that SL biosynthesis and signaling pathway genes are conserved, but it also revealed that SL biosynthesis gene GmD27c and legume rhizobia have close interactions in controlling plant nodule number.

Indexed as

Glycine maxRhizobiumGene Expression Regulation, PlantHeterocyclic Compounds, 3-RingLactonesPhosphatesPhylogenyPlant ProteinsGR24 strigolactoneHeterocyclic Compounds, 3-RingLactonesPhosphatesPlant ProteinsExpression profilesGmD27 gene familyRhizobia infectionSoybean hairy rootStrigolactones

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PMID35025033
OpenAlexW4205758675

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.