Evidence map›Paper›PMID 35024916›Full record

ArticleAnalytical and bioanalytical chemistry2022

A photonic crystal fiber-based fluorescence sensor for simultaneous and sensitive detection of lactic acid enantiomers.

Zhongmei Chi, Minmin Li, Jia Xu, Li Yang

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Zhongmei ChiKey Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, 130024, Jilin, China.
Minmin LiKey Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, 130024, Jilin, China.
Jia XuKey Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, 130024, Jilin, China.
Li YangKey Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, 130024, Jilin, China. yangl330@nenu.edu.cn.
Northeast Normal University · CNBohai University · CN

Funding

National Natural Science Foundation of China 21775017/22074014Natural Science Foundation of Jilin Province 20200404153YY
6 · The paper itself

Abstract

A photonic crystal fiber (PCF)-based fluorescence sensor is developed for rapid and sensitive detection of lactic acid (LA) enantiomers in serum samples. The sensor is fabricated by chemical binding dual enzymes on the inner surface of the PCF with numerous pore structures and a large specific surface area, which is suitable to be utilized as an enzymatic reaction carrier. To achieve simultaneous detection of L-LA and D-LA, the PCF with an aldehyde-activated surface is cut into two separate pieces, one of which is coated with L-LDH/GPT enzymes and the other with D-LDH/GPT enzymes. By being connected and carefully aligned to each other by a suitable sleeve tube connector, the responses of both L-LA and D-LA sensors are determined by laser-induced flourescence (LIF) detection. With the aid of enzyme-linked catalytic reactions, the proposed PCF sensor can greatly improve the sensitivity and analysis speed for the detection of LA enantiomers. The PCF sensor exhibits a low limit of detection of 9.5 μM and 0.8 μM, and a wide linear range of 25-2000 μM and 2-400 μM for L-LA and D-LA, respectively. The sensor has been successfully applied to accurate determination of LA enantiomers in human serum with satisfactory reproducibility and stability. It is indicated that the present PCF sensors would be used as an attractive analytical platform for quantitative detection of trace-amount LA enantiomers in real biological samples, and thus would play a role in disease diagnosis and clinical monitoring in point-of-care testing.

Indexed as

Enzymes, ImmobilizedEquipment DesignFluorescenceHumansLactic AcidLasersLimit of DetectionL-Lactate DehydrogenaseMicroscopy, Electron, ScanningOptics and PhotonicsReproducibility of ResultsSensitivity and SpecificitySpectroscopy, Fourier Transform InfraredStereoisomerismEnzymes, ImmobilizedLactic AcidL-Lactate DehydrogenaseEnzyme-linked catalytic reactionFluorescence sensorLactic acid enantiomersPhotonic crystal fiber

Identifiers

PMID35024916
PMCPMC8756416
OpenAlexW4205915808

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.