Evidence map›Paper›PMID 35023082›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2022

Analysis of Gtpases Rab 5 and Rab 7 expression from macrophages infected with biofilm-producing and non biofilm-producing strains of Corynebacterium pseudotuberculosis.

Maria da Conceição Aquino de Sá, José Tadeu Raynal Rocha Filho, Maria Emilia Alcantara, Marcos da Costa Silva, Mariane Melo Dos Santos, Allan Souza Dos Santos, Mateus Matiuzzi da Costa, Roberto Meyer

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In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Maria da Conceição Aquino de SáLaboratory of Immunology and Molecular Biology, Institute of Health Sciences - Federal University of Bahia, Salvador, BA, Brazil. ceicazoo@hotmail.com.ORCID http://orcid.org/0000-0001-8145-7018
José Tadeu Raynal Rocha FilhoLaboratory of Immunology and Molecular Biology, Institute of Health Sciences - Federal University of Bahia, Salvador, BA, Brazil.
Maria Emilia AlcantaraLaboratory of Immunology and Molecular Biology, Institute of Health Sciences - Federal University of Bahia, Salvador, BA, Brazil.
Marcos da Costa SilvaBahia State University, Salvador, BA, Brazil.
Mariane Melo Dos SantosLaboratory of Immunology and Molecular Biology, Institute of Health Sciences - Federal University of Bahia, Salvador, BA, Brazil.
Allan Souza Dos SantosLaboratory of Immunology and Molecular Biology, Institute of Health Sciences - Federal University of Bahia, Salvador, BA, Brazil.
Mateus Matiuzzi da CostaFederal University of Vale Do São Francisco, Petrolina, PE, Brazil.
Roberto MeyerLaboratory of Immunology and Molecular Biology, Institute of Health Sciences - Federal University of Bahia, Salvador, BA, Brazil.
Universidade Federal da Bahia · BRUniversidade do Estado da Bahia · BRUniversidade Federal do Vale do São Francisco · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Corynebacterium pseudotuberculosis is a facultative intracellular pathogen that uses various mechanisms to survive within macrophages. In phagocytosis, this survival can be attributed to the ability to inhibit phagosome-lysosome fusion. In this fusion, some proteins, including Rabs GTPases, are involved in the maturation process and are responsible for regulating membrane vesicle trafficking. Thus, to better understand these mechanisms, the capacity of biofilm-producing and non biofilm-producing strains of Corynebacterium pseudotuberculosis for modulating the expression of endosomal proteins GTPases Rab 5 and Rab 7 was evaluated in an in vitro study of infection of goat macrophages. Blood was collected from ten Canindé goats, infected with biofilm-producing and non biofilm-producing strains of C. pseudotuberculosis. Blood cells were separated in colloidal silica-polyvinylpyrrolidone gradients (GE Healthcare®). These cells were maintained at 37 °C, with 5% of CO

Indexed as

Corynebacterium InfectionsCorynebacterium pseudotuberculosisAnimalsBiofilmsMacrophagesPhagocytosisrab GTP-Binding Proteinsrab GTP-Binding ProteinsBiofilmFlow cytometryRab 5 and Rab 7 proteins

Identifiers

PMID35023082
PMCPMC8882527
OpenAlexW4205723057

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.