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ArticleMolecular biology reports2022

Mitogenome of Knodus borki (Cypriniformes: Characidae): genomic characterization and phylogenetic analysis.

Cheng-He Sun, Ya-Nan Zhang, Xiao-Shu Zeng, Da-Wei Liu, Qi Huang, Xiao-Li Zhang, Qun Zhang

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Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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7 authors.

Cheng-He SunDepartment of Ecology and Institute of Hydrobiology, Jinan University, Guangzhou, 510632, China.ORCID http://orcid.org/0000-0002-0650-5443
Ya-Nan ZhangDepartment of Ecology and Institute of Hydrobiology, Jinan University, Guangzhou, 510632, China.
Xiao-Shu ZengDepartment of Ecology and Institute of Hydrobiology, Jinan University, Guangzhou, 510632, China.
Da-Wei LiuForest Police Identification Center of National Forestry Administration, Nanjing Forest Police College, Nanjing, 210023, China.
Qi HuangDepartment of Ecology and Institute of Hydrobiology, Jinan University, Guangzhou, 510632, China.
Xiao-Li ZhangDepartment of Ecology and Institute of Hydrobiology, Jinan University, Guangzhou, 510632, China.
Qun ZhangDepartment of Ecology and Institute of Hydrobiology, Jinan University, Guangzhou, 510632, China. tqzhang@jnu.edu.cn.ORCID http://orcid.org/0000-0003-3885-1540

Funding

The National Key R&D Program of China 2018YFD0900802The Open Project of Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes SXSN/4498The Outstanding Innovative Talents Cultivation Funded Programs for Doctoral Students of Jinan University 2021CXB022
6 · The paper itself

Abstract

backgroundThe taxonomic status of Knodu in the family Characidae is not yet clear. This study aimed to address this by sequencing and annotating Knodu borki Zarske, 2008. MATERIALS AND

resultsK. borki Zarske, 2008 was sequenced using a Hiseq platform and the complete mitogenome was assembled in SPAdes v3.15.2 and SOAPdenovo2 v.2.01. The mitogenome of K. borki from Guangzhou, the first sequenced species of the genus Knodu, is 16,837 bp in length and contains 13 protein-coding genes (PCGs), two ribosomal (r) RNAs, 22 transfer (t) RNAs, and one D-loop. Among these 37 genes, 28 are encoded by the heavy strand, while nine are encoded by the light strand. Twenty-one of the tRNAs can form typical cloverleaf secondary structures, except tRNA-Ser1, which lacks dihydrouridine arms. All PCGs have the same start codon (ATG), with the exception of COI (GTG). Four PCGs (ND1, ATP8, ND4L, and ND5) have TAA as the stop codon, ND6 has TAG as the stop codon, COI has AGG as the stop codon, and the remaining seven genes have incomplete stop codons of TA-/T-(ND2, COII, COIII, ND3, ND4, and Cyt b as T-, ATP6 as TA-). Phylogenetic analysis showed that K. borki belongs to the family Characidae.

conclusionsOur findings demonstrate that K. borki belongs to the family Characidae, due to consistency with the morphological identification. This study provides molecular information for further research on the phylogeny of the genus Knodus and for analyses of the taxonomic status of Characidae.

Indexed as

CharacidaeCypriniformesGenome, MitochondrialAnimalsBase CompositionGenomicsPhylogenyRNA, RibosomalRNA, TransferSequence Analysis, DNARNA, RibosomalRNA, TransferCharacidaeKnodus borkiMitochondrial genomePhylogeny

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