Evidence map›Paper›PMID 35021313›Full record

ArticleThe world journal of men's health2022

Therapeutic Effect of Human Mesenchymal Stem Cell-Conditioned Medium on Erectile Dysfunction.

Seul Gi Kim, Dalsan You, Kyung Kim, Joomin Aum, Yu Seon Kim, Myoung Jin Jang, Kyung Hyun Moon, Hyun-Wook Kang

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The world journal of men's health, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Seul Gi Kim *Department of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan, Korea.ORCID https://orcid.org/0000-0003-3824-2438
Dalsan You *Department of Urology, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.ORCID https://orcid.org/0000-0001-8152-847X
Kyung KimDepartment of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.ORCID https://orcid.org/0000-0001-8034-7116
Joomin AumDepartment of Urology, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.ORCID https://orcid.org/0000-0001-9078-1012
Yu Seon KimDepartment of Urology, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.ORCID https://orcid.org/0000-0002-0390-5062
Myoung Jin JangAsan Institute for Life Sciences, Asan Medical Center, Seoul, Korea.ORCID https://orcid.org/0000-0001-8123-5001
Kyung Hyun MoonDepartment of Urology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea. urofirst@uuh.ulsan.kr.ORCID https://orcid.org/0000-0002-5966-0025
Hyun-Wook KangDepartment of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan, Korea. hkang@unist.ac.kr.ORCID https://orcid.org/0000-0001-5335-5604
Ulsan College · KRUlsan National Institute of Science and Technology · KRAsan Medical Center · KR

Funding

Asan Institute for Life Sciences, Asan Medical Center 2019-0576National Research Foundation of Korea NRF-2018K1A4A3A01063890National Research Foundation of Korea NRF-2020H1D3A2A01110944National Research Foundation of Korea NRF-2021R1A2C2012808
6 · The paper itself

Abstract

purposeOwing to the safety and cost effectiveness of conditioned medium (CM), its therapeutic effects have attracted significant attention from many researchers. To date, numerous studies have been conducted on CM; however, little has been done with regard to erectile dysfunction (ED). In this research, the potential of human mesenchymal stem cell-derived CM (MSC-CM) for the treatment of ED was investigated. MATERIALS AND

methodsA high concentration of MSC-CM was prepared through 3D spheroid culturing with bone marrow-derived MSCs and cut-off filtering. The composition of CM was analyzed using biochemical assays, and the effect of the preparation process on the quality of CM was investigated. The therapeutic effects of MSC-CM were evaluated through animal studies using a cavernous nerve (CN) injury rat model.

results3D spheroid culturing afforded a 278-fold increase in the total protein content of CM, as compared to that from 2D cultures; the protein concentration increased by 19 times on increasing the centrifugation time for cut-off filtering. Biochemical assays indicated that the CM contains various types of angiogenic, neurotrophic, and anti-inflammatory factors. Histological assay results showed that MSC-CM has angio- and neuro-trophic effect in a CN injury rat model

conclusionsThe experimental results confirmed the therapeutic effect of MSC-CM in healing damaged cavernosal tissue and restoring erectile function. These results successfully demonstrated that MSC-CM has significant potential for the treatment of ED.

Indexed as

Angiotrophic effectConditioned mediumErectile dysfunctionMesenchymal stem cellNeurotrophic effect

Identifiers

PMID35021313
PMCPMC9482857
OpenAlexW4205369735

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.