ArticleDevelopment (Cambridge, England)2021
In vivo proximity biotin ligation identifies the interactome of Egalitarian, a Dynein cargo adaptor.
Article in Development (Cambridge, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- AnbioRxiv : the preprint server for biology · 2026Article
- The Cappuccino interactome reveals an intracellular role for Semaphorin-2a inbioRxiv : the preprint server for biology · 2026Article
- Bridges under construction: the dynamics of ring canal expansion during Drosophila oogenesis.Developmental biology · 2026Review
- The Rab7-Epg5 and Rab39-ema modules cooperatively position autophagosomes for efficient lysosomal fusions.eLife · 2025Article
- Article
- The role of ER exit sites in maintaining P-body organization and integrity during Drosophila melanogaster oogenesis.EMBO reports · 2025Article
- The mRNA dynamics underpinning translational control mechanisms of Drosophila melanogaster oogenesis.Biochemical Society transactions · 2024Review
- The role of ER exit sites in maintaining P-body organization and transmitting ER stress response duringbioRxiv : the preprint server for biology · 2024Article
- Transcriptional signature of host shift in the seed beetle Zabrotes subfasciatus.Genetics and molecular biology · 2024Article
- dTtc1, a conserved tetratricopeptide repeat protein, is required for maturation ofFrontiers in cell and developmental biology · 2023Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Numerous motors of the Kinesin family contribute to plus-end-directed microtubule transport. However, almost all transport towards the minus-end of microtubules involves Dynein. Understanding the mechanism by which Dynein transports this vast diversity of cargo is the focus of intense research. In selected cases, adaptors that link a particular cargo with Dynein have been identified. However, the sheer diversity of cargo suggests that additional adaptors must exist. We used the Drosophila egg chamber as a model to address this issue. Within egg chambers, Egalitarian is required for linking mRNA with Dynein. However, in the absence of Egalitarian, Dynein transport into the oocyte is severely compromised. This suggests that additional cargoes might be linked to Dynein in an Egalitarian-dependent manner. We therefore used proximity biotin ligation to define the interactome of Egalitarian. This approach yielded several novel interacting partners, including P body components and proteins that associate with Dynein in mammalian cells. We also devised and validated a nanobody-based proximity biotinylation strategy that can be used to define the interactome of any GFP-tagged protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.