ArticleMolecular biology of the cell2022
Nanoscale dynamics of actin filaments in the red blood cell membrane skeleton.
Article in Molecular biology of the cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 24 citations in OpenAlex.
- The membrane skeleton density of red blood cells in MYH9-related disease patients is decreased.Bioscience reports · 2026Article
- Active Force Dynamics in Red Blood Cells Under Non-Invasive Optical Tweezers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Pair cross-correlation analysis for assessing protein co-localization.Biophysical journal · 2025Article
- Subcellular Mechanical Imaging of Erythrocytes with Optically Correlated Scanning Ion Conductance Microscopy.ACS measurement science au · 2025Article
- Dynamic mechanisms for membrane skeleton transitions.Journal of cell science · 2025Article
- Stomatocyte-discocyte-echinocyte transformations of erythrocyte modulated by membrane-cytoskeleton mechanical properties.Biophysical journal · 2025Article
- Fascial Manual Medicine: A Continuous Evolution.Cureus · 2024Review
- Mechanically induced topological transition of spectrin regulates its distribution in the mammalian cell cortex.Nature communications · 2024Article
- Dynamic mechanisms for membrane skeleton transitions.bioRxiv : the preprint server for biology · 2024Article
- Effective cell membrane tension protects red blood cells against malaria invasion.PLoS computational biology · 2023Article
- Tank-treading dynamics of red blood cells in shear flow: On the membrane viscosity rheology.Biophysical journal · 2022Article
- A particle-based computational model to analyse remodelling of the red blood cell cytoskeleton during malaria infections.PLoS computational biology · 2022Article
- Structural Configuration of Blood Cell Membranes Determines Their Nonlinear Deformation Properties.BioMed research international · 2022Article
- Mechanical Principles Governing the Shapes of Dendritic Spines.Frontiers in physiology · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
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Abstract
Red blood cell (RBC) shape and deformability are supported by a planar network of short actin filament (F-actin) nodes (∼37 nm length, 15-18 subunits) interconnected by long spectrin strands at the inner surface of the plasma membrane. Spectrin-F-actin network structure underlies quantitative modeling of forces controlling RBC shape, membrane curvature, and deformation, yet the nanoscale organization and dynamics of the F-actin nodes in situ are not well understood. We examined F-actin distribution and dynamics in RBCs using fluorescent-phalloidin labeling of F-actin imaged by multiple microscopy modalities. Total internal reflection fluorescence and Zeiss Airyscan confocal microscopy demonstrate that F-actin is concentrated in multiple brightly stained F-actin foci ∼200-300 nm apart interspersed with dimmer F-actin staining regions. Single molecule stochastic optical reconstruction microscopy imaging of Alexa 647-phalloidin-labeled F-actin and computational analysis also indicates an irregular, nonrandom distribution of F-actin nodes. Treatment of RBCs with latrunculin A and cytochalasin D indicates that F-actin foci distribution depends on actin polymerization, while live cell imaging reveals dynamic local motions of F-actin foci, with lateral movements, appearance and disappearance. Regulation of F-actin node distribution and dynamics via actin assembly/disassembly pathways and/or via local extension and retraction of spectrin strands may provide a new mechanism to control spectrin-F-actin network connectivity, RBC shape, and membrane deformability.
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