Evidence map›Paper›PMID 35019667›Full record

ArticlemSphere2022

Efficient Gene Knockout and Knockdown Systems in Neospora caninum Enable Rapid Discovery and Functional Assessment of Novel Proteins.

Tiago W P Mineo, Jessica H Chern, Amara C Thind, Caroline M Mota, Santhosh M Nadipuram, Juan A Torres, Peter J Bradley

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mSphere, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.6field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Microorganisms · 2026
    Review
  2. Article
  3. Article
  4. ThemSphere · 2023
    Article
  5. Article
  6. Frontiers in microbiology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

Tiago W P MineoDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angelesgrid.19006.3e, Los Angeles, California, USA.ORCID 0000-0002-2339-2743
Jessica H ChernDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angelesgrid.19006.3e, Los Angeles, California, USA.
Amara C ThindDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angelesgrid.19006.3e, Los Angeles, California, USA.
Caroline M MotaDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angelesgrid.19006.3e, Los Angeles, California, USA.
Santhosh M NadipuramDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angelesgrid.19006.3e, Los Angeles, California, USA.
Juan A TorresDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angelesgrid.19006.3e, Los Angeles, California, USA.
Peter J BradleyDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angelesgrid.19006.3e, Los Angeles, California, USA.ORCID 0000-0002-8481-0638
University of California, Los Angeles · US

Funding

MARC U*STAR Program at the University of California, Los AngelesT34GM008563 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MCEVOY, MEGAN M, POE, GINA R · 1996 to 2023
$9.7M
MULTIDISCIPLINARY TRAINING IN MICROBIAL PATHOGENESIST32AI007323 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Peter John Bradley · 1988 to 2026
$8.6M
Functional Analysis of Novel Components of the Toxoplasma Inner Membrane ComplexR01AI123360 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Peter John Bradley · 2017 to 2026
$3.8M
The Role of Toxoplasma Rhoptries in Host Cell InfectionR01AI064616 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BRADLEY, PETER JOHN · 2006 to 2016
$3.5M
NIAID NIH HHS R01 AI064616NIAID NIH HHS R01 AI123360NIAID NIH HHS T32 AI007323NIGMS NIH HHS T34 GM008563
6 · The paper itself

Abstract

The development of molecular genetics has greatly enhanced the study of the biology and pathology associated with parasites of the phylum Apicomplexa. While the molecular tools are highly developed for the apicomplexan Toxoplasma gondii, the closely related parasite Neospora caninum lacks efficient tools for genetic manipulation. To enable efficient homologous recombination in N. caninum, we targeted the Ku heterodimer DNA repair mechanism in the genomic reference strain, Nc-Liverpool (NcLiv), and show that deletion of

Indexed as

NeosporaToxoplasmaAnimalsCattleDogsGene Knockout TechniquesPhylogenyReproductionauxin-inducible degronCRISPR/Cas9dense granuleKu80Neospora caninumToxoplasma gondii

Identifiers

PMID35019667
PMCPMC8754167
OpenAlexW4206319503

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.