ArticleThe Plant cell2022
A cascade of bHLH-regulated pathways programs maize anther development.
Article in The Plant cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Presence and function of small RNAs during plant reproduction.RNA biology · 2026Review
- The SbTDR gene is required for anther development and male fertility in Sorghum bicolor.Plant reproduction · 2026Article
- Multi-Tissue Transcriptomic Profiling Identifies Anther Preferentially Expressed Candidate Genes in Cotton.Biology · 2026Article
- The Maize ZmbHLH118 Transcription Factor Regulates Vacuolar Nitrate Loading by the NOAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Transcriptional reprogramming of heat-sensitive maize anther development under heat stress.BMC plant biology · 2026Article
- Maize Anther Development Involves Translated Open Reading Frames From 3' Untranslated Regions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The bHLH transcription factor OsbHLH148 negatively regulates rice tiller number and grain yield through an integrated transcriptional network.BMC genomics · 2026Article
- Reproductive phasiRNAs are the piRNAs of plants.Trends in genetics : TIG · 2026Review
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- bHLH Transcription Factors in Cereal Crops: Diverse Functions in Regulating Growth, Development and Stress Responses.International journal of molecular sciences · 2025Review
- The basic helix-loop-helix (bHLH) transcription factor DTT1 is part of a paired key that unlocks the tapetum transition in barley anther development.The Plant cell · 2025Article
- Article
- Targeted seed EMS mutagenesis reveals a basic helix-loop-helix transcription factor underlying male sterility in sorghum.Genetics · 2025Article
- Fine mapping and candidate gene analysis of major QTLs for number of seeds per pod in Arachis hypogaea L.BMC genomics · 2025Article
- ExPOSE: a comprehensive toolkit to perform expansion microscopy in plant protoplast systems.The Plant journal : for cell and molecular biology · 2025Article
- Article
- Genome-wide association mapping and genomic prediction analyses reveal the genetic architecture of grain yield and agronomic traits under drought and optimum conditions in maize.BMC plant biology · 2025Article
- Article
- Ribosome binding of phasiRNA precursors accelerates the 24-nt phasiRNA burst in meiotic maize anthers.The Plant cell · 2024Article
- Genome-Wide Analysis ofInternational journal of molecular sciences · 2024Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spatiotemporal development of somatic tissues of the anther lobe is necessary for successful fertile pollen production. This process is mediated by many transcription factors acting through complex, multi-layered networks. Here, our analysis of functional knockout mutants of interacting basic helix-loop-helix genes Ms23, Ms32, basic helix-loop-helix 122 (bHLH122), and bHLH51 in maize (Zea mays) established that male fertility requires all four genes, expressed sequentially in the tapetum (TP). Not only do they regulate each other, but also they encode proteins that form heterodimers that act collaboratively to guide many cellular processes at specific developmental stages. MS23 is confirmed to be the master factor, as the ms23 mutant showed the earliest developmental defect, cytologically visible in the TP, with the most drastic alterations in premeiotic gene expression observed in ms23 anthers. Notably, the male-sterile ms23, ms32, and bhlh122-1 mutants lack 24-nt phased secondary small interfering RNAs (phasiRNAs) and the precursor transcripts from the corresponding 24-PHAS loci, while the bhlh51-1 mutant has wild-type levels of both precursors and small RNA products. Multiple lines of evidence suggest that 24-nt phasiRNA biogenesis primarily occurs downstream of MS23 and MS32, both of which directly activate Dcl5 and are required for most 24-PHAS transcription, with bHLH122 playing a distinct role in 24-PHAS transcription.
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