Evidence map›Paper›PMID 35017838›Full record

ArticleVeterinary world2021

Expression of the recombinant C-terminal of the S1 domain and N-terminal of the S2 domain of the spike protein of porcine epidemic diarrhea virus.

Jiraporn Sritun, Natnaree Inthong, Siriluk Jala, Sakuna Phatthanakunanan, Khomson Satchasataporn, Kaitkanoke Sirinarumitr, Preeda Lertwatcharasarakul, Theerapol Sirinarumitr

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In one paragraph

Article in Veterinary world, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Jiraporn SritunBio-Veterinary Sciences Program, Faculty of Veterinary Medicine, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok, 10900, Thailand.
Natnaree InthongDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok, 10900, Thailand.
Siriluk JalaKamphaeng Saen Veterinary Diagnosis Center, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom, 73140, Thailand.
Sakuna PhatthanakunananKamphaeng Saen Veterinary Diagnosis Center, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom, 73140, Thailand.
Khomson SatchasatapornDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok, 10900, Thailand.
Kaitkanoke SirinarumitrDepartment of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok, 10900, Thailand.
Preeda LertwatcharasarakulDepartment of Pathology, Faculty of Veterinary Medicine, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok, 10900, Thailand.
Theerapol SirinarumitrDepartment of Pathology, Faculty of Veterinary Medicine, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok, 10900, Thailand.
Kasetsart University · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

aimPorcine epidemic diarrhea virus (PEDV) causes severe diarrhea in suckling piglets, leading to severe economic losses in the swine industry. Commercial vaccines have limited effectiveness against different genogroups of PEDV and the shedding of virus. The C-terminal of the S1 domain and the N-terminal of the S2 domain (S1-2) protein of the spike (S) protein have four neutralizing epitopes. However, research on the expression of the S1-2 segment of the S gene has been limited. In this study, we expressed a recombinant S1-2 protein of the S protein of the PEDV Thai isolate and characterized the immunological properties of the recombinant S1-2 protein. MATERIALS AND

methodsThe S1-2 segment of the S gene of the PEDV Thai isolate (G2b) was amplified, cloned into the pBAD202/D-TOPO

resultsThe recombinant S1-2 segment of the S gene of the PEDV Thai isolate protein was cloned and the recombinant S1-2 protein was successfully expressed. The optimum concentration of arabinose and the optimum induction time for the induction of the recombinant S1-2 protein were 0.2% and 8 h, respectively. The recombinant S1-2 protein reacted specifically with both rabbit anti-histidine polyclonal antibodies and rabbit anti-PEDV polyclonal antibodies.

conclusionThe recombinant S1-2 protein reacted with rabbit anti-PEDV polyclonal antibodies induced by the different PEDV genogroup. Therefore, the recombinant S1-2 protein may be a useful tool for the development of a diagnostic test for PEDV or for a vaccine against PEDV.

Indexed as

diagnosticsgenogroupporcine epidemic diarrhea virusrecombinant proteinvaccine

Identifiers

PMID35017838
PMCPMC8743769
OpenAlexW3212756350

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.