ArticleScientific reports2022
3D nanomechanical mapping of subcellular and sub-nuclear structures of living cells by multi-harmonic AFM with long-tip microcantilevers.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- High-resolution nuclear cell biology by cryo-electron tomography.Nucleus (Austin, Tex.) · 2026Review
- Optimal design for multi-compartment cell elasticity estimation using AFM and super-resolution imaging.Computer methods and programs in biomedicine · 2026Article
- Advances in nanomechanical property mapping by atomic force microscopy.Nanoscale advances · 2025Review
- Updates on the Advantages and Disadvantages of Microscopic and Spectroscopic Characterization of Magnetotactic Bacteria for Biosensor Applications.Biosensors · 2025Review
- Cell spheroid micromechanics under large deformations.Scientific reports · 2025Article
- Nonlinear Harmonics: A Gateway to Enhanced Image Contrast and Material Discrimination.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Altering the mechanical properties of self-assembled filaments through engineering of EspA bacterial protein.Materials today. Bio · 2025Article
- Size Matters: Rethinking Hertz Model Interpretation for Cell Mechanics Using AFM.International journal of molecular sciences · 2024Article
- Holotomography and atomic force microscopy: a powerful combination to enhance cancer, microbiology and nanotoxicology research.Discover nano · 2024Review
- Simulating biological surface dynamics in high-speed atomic force microscopy experiments.Biophysical reviews · 2023Article
- Article
- Imaging beyond the surface region: Probing hidden materials via atomic force microscopy.Science advances · 2023Review
- Thermal Treatment Impact on the Mechanical Properties of MgMaterials (Basel, Switzerland) · 2022Article
- Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.Progress in biophysics and molecular biology · 2022Review
- Advances in Cancer Diagnosis: Bio-Electrochemical and Biophysical Characterizations of Cancer Cells.Micromachines · 2022Review
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Authors and funding
4 authors.
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Abstract
Recent developments such as multi-harmonic Atomic Force Microscopy (AFM) techniques have enabled fast, quantitative mapping of nanomechanical properties of living cells. Due to their high spatiotemporal resolution, these methods provide new insights into changes of mechanical properties of subcellular structures due to disease or drug response. Here, we propose three new improvements to significantly improve the resolution, identification, and mechanical property quantification of sub-cellular and sub-nuclear structures using multi-harmonic AFM on living cells. First, microcantilever tips are streamlined using long-carbon tips to minimize long-range hydrodynamic interactions with the cell surface, to enhance the spatial resolution of nanomechanical maps and minimize hydrodynamic artifacts. Second, simultaneous Spinning Disk Confocal Microscopy (SDC) with live-cell fluorescent markers enables the unambiguous correlation between observed heterogeneities in nanomechanical maps with subcellular structures. Third, computational approaches are then used to estimate the mechanical properties of sub-nuclear structures. Results are demonstrated on living NIH 3T3 fibroblasts and breast cancer MDA-MB-231 cells, where properties of nucleoli, a deep intracellular structure, were assessed. The integrated approach opens the door to study the mechanobiology of sub-cellular structures during disease or drug response.
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