ArticlePlant biotechnology journal2022
Development of an efficient marker-free soybean transformation method using the novel bacterium Ochrobactrum haywardense H1.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 40 citations in OpenAlex.
- Targeted allelic diversification of flowering genes via CRISPR-Cas enables the development of multiple early-flowering soybean lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Developmental regulators enable rapid and efficient soybean transformation and CRISPR-mediated genome editing.Plant physiology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Genotype-flexible plant genetic transformation: advances and prospects.Frontiers in plant science · 2026Review
- CRISPR-Based genome editing in pulses: current approaches, challenges, and future prospects.Plant molecular biology · 2025Review
- Co-transformation using T-DNA genes from Agrobacterium strain 82.139 enhances regeneration of transgenic shoots in Populus.Plant biotechnology journal · 2025Article
- Engineering Agrobacterium for improved plant transformation.The Plant journal : for cell and molecular biology · 2025Review
- Crown Gall Induced by a Natural Isolate ofMicroorganisms · 2025Article
- Development of high-throughput tissue culture-free plant transformation systems.The Plant journal : for cell and molecular biology · 2025Review
- A fast and genotype-independent in planta Agrobacterium-mediated transformation method for soybean.Plant communications · 2024Article
- Soybean genomics research community strategic plan: A vision for 2024-2028.The plant genome · 2024Article
- Advances in Soybean Genetic Improvement.Plants (Basel, Switzerland) · 2024Review
- A Novel Soybean Diacylglycerol Acyltransferase 1b Variant with Three Amino Acid Substitutions Increases Seed Oil Content.Plant & cell physiology · 2024Article
- A comprehensive review of in planta stable transformation strategies.Plant methods · 2024Review
- EnhancingFrontiers in plant science · 2024Article
- Aztreonam is a novel chemical inducer that promotesFrontiers in microbiology · 2023Article
- CRISPR/LbCas12a-Mediated Genome Editing in Soybean.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Recalcitrance to transformation, a hindrance for genome editing of legumes.Frontiers in genome editing · 2023Review
- Article
- Efficient gene targeting in soybean using Ochrobactrum haywardense-mediated delivery of a marker-free donor template.Plant physiology · 2022Article
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have discovered a novel bacterium, Ochrobactrum haywardense H1 (Oh H1), which is capable of efficient plant transformation. Ochrobactrum is a new host for Agrobacterium-derived vir and T-DNA-mediated transformation. Oh H1 is a unique, non-phytopathogenic species, categorized as a BSL-1 organism. We engineered Oh H1 with repurposed Agrobacterium virulence machinery and demonstrated Oh H1 can transform numerous dicot species and at least one monocot, sorghum. We generated a cysteine auxotrophic Oh H1-8 strain containing a binary vector system. Oh H1-8 produced transgenic soybean plants with an efficiency 1.6 times that of Agrobacterium strain AGL1 and 2.9 times that of LBA4404Thy-. Oh H1-8 successfully transformed several elite Corteva soybean varieties with T0 transformation frequency up to 35%. In addition to higher transformation efficiencies, Oh H1-8 generated high-quality, transgenic events with single-copy, plasmid backbone-free insertion at frequencies higher than AGL1. The SpcN selectable marker gene is excised using a heat shock-inducible excision system resulting in marker-free transgenic events. Approximately, 24.5% of the regenerated plants contained only a single copy of the transgene and contained no vector backbone. There were no statistically significant differences in yield comparing T3 null-segregant lines to wild-type controls. We have demonstrated that Oh H1-8, combined with spectinomycin selection, is an efficient, rapid, marker-free and yield-neutral transformation system for elite soybean.
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