Evidence map›Paper›PMID 35014949›Full record

ArticleMicrobial genomics2022

Hackflex: low-cost, high-throughput, Illumina Nextera Flex library construction.

Daniela Gaio, Kay Anantanawat, Joyce To, Michael Liu, Leigh Monahan, Aaron E Darling

Open access · goldAbstract read
In one paragraph

Article in Microbial genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.

0numbers the graph read from it
0cells of the map it votes in
63citing papers in PubMed
6.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

63 citing papers in PubMed, 89 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Multicenter Genomic Analysis of Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2026
    Article
  5. Evolutionary adaptations ofMicrobiology spectrum · 2026
    Article
  6. Article
  7. Profiling ofMicrobiology spectrum · 2026
    Article
  8. Complete genome of aMicrobiology resource announcements · 2026
    Article
  9. Article
  10. Article
  11. Article
  12. The R plasmid pRAS2 in the fish pathogenFrontiers in microbiology · 2026
    Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article

3 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Daniela GaioiThree Institute, University of Technology Sydney, Sydney, NSW, Australia.
Kay AnantanawatiThree Institute, University of Technology Sydney, Sydney, NSW, Australia.
Joyce ToiThree Institute, University of Technology Sydney, Sydney, NSW, Australia.
Michael LiuiThree Institute, University of Technology Sydney, Sydney, NSW, Australia.
Leigh MonahaniThree Institute, University of Technology Sydney, Sydney, NSW, Australia.
Aaron E DarlingiThree Institute, University of Technology Sydney, Sydney, NSW, Australia.
University of Technology Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a low-cost method for the production of Illumina-compatible sequencing libraries that allows up to 14 times more libraries for high-throughput Illumina sequencing to be generated for the same cost. We call this new method Hackflex. The quality of library preparation was tested by constructing libraries from

Indexed as

Gene LibraryAustraliaBacteriaBase CompositionDNA, BacterialEscherichia coliHigh-Throughput Nucleotide SequencingPseudomonas aeruginosaSequence Analysis, DNAStaphylococcus aureusDNA, Bacterialhigh-throughput sequencingIllumina sequencinglibrary preparationmetagenomicsmultiplexNextera Flex

Identifiers

PMID35014949
PMCPMC8914357
OpenAlexW4205280965

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.