Evidence map›Paper›PMID 35014670›Full record

ArticleThe Journal of experimental biology2022

An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos.

Predrag Jevtić, K Wade Elliott, Shelby E Watkins, Jonathan A Sreter, Katarina Jovic, Ian B Lehner, Paul W Baures, John G Tsavalas, Daniel L Levy, Krisztina Varga

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of experimental biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Predrag JevtićDepartment of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA.
K Wade ElliottDepartment of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH 03824, USA.ORCID 0000-0001-9442-0935
Shelby E WatkinsDepartment of Chemistry, University of Wyoming, Laramie, WY 82071, USA.
Jonathan A SreterDepartment of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH 03824, USA.ORCID 0000-0001-9562-741X
Katarina JovicDepartment of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH 03824, USA.ORCID 0000-0002-8838-7929
Ian B LehnerDepartment of Chemistry, Keene State College, Keene, NH 03435, USA.
Paul W BauresDepartment of Chemistry, Keene State College, Keene, NH 03435, USA.ORCID 0000-0003-3879-8428
John G TsavalasDepartment of Chemistry, University of New Hampshire, Durham, NH 03824, USA.ORCID 0000-0003-4955-9991
Daniel L LevyDepartment of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA.ORCID 0000-0002-7853-3275
Krisztina VargaDepartment of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH 03824, USA.ORCID 0000-0003-2810-0997
University of New Hampshire · USUniversity of Wyoming · USKeene State College · US

Funding

Wyoming INBRE Phase 4- Equipment Supplement for x-ray diffractometer for Center for Advanced Scientific InstrumentationP20GM103432 · NIGMS · UNIVERSITY OF WYOMING · PI Nicolas A. Blouin · 2012 to 2026
$56.8M
Mechanisms of nuclear size regulationR35GM134885 · NIGMS · UNIVERSITY OF WYOMING · PI Daniel Leon Levy · 2020 to 2026
$2.5M
The Structure-Activity Relationship of a Macrocycle with Antifreeze PropertiesR15GM135903 · NIGMS · KEENE STATE COLLEGE · PI BAURES, PAUL W. · 2020 to 2020
$376k
NASA 80NSSC18M0034NIGMS NIH HHS P20 GM103432NIGMS NIH HHS R15 GM135903NIGMS NIH HHS R35 GM134885NIH HHS GM135903
6 · The paper itself

Abstract

Cryoprotection is of interest in many fields of research, necessitating a greater understanding of different cryoprotective agents. Antifreeze proteins have been identified that have the ability to confer cryoprotection in certain organisms. Antifreeze proteins are an evolutionary adaptation that contributes to the freeze resistance of certain fish, insects, bacteria and plants. These proteins adsorb to an ice crystal's surface and restrict its growth within a certain temperature range. We investigated the ability of an antifreeze protein from the desert beetle Anatolica polita, ApAFP752, to confer cryoprotection in the frog Xenopus laevis. Xenopus laevis eggs and embryos microinjected with ApAFP752 exhibited reduced damage and increased survival after a freeze-thaw cycle in a concentration-dependent manner. We also demonstrate that ApAFP752 localizes to the plasma membrane in eggs and embryonic blastomeres and is not toxic for early development. These studies show the potential of an insect antifreeze protein to confer cryoprotection in amphibian eggs and embryos.

Indexed as

Antifreeze ProteinsColeopteraEmbryo, NonmammalianInsect ProteinsOvumAnimalsCryoprotective AgentsXenopus laevisAntifreeze ProteinsCryoprotective AgentsInsect ProteinsAntifreeze proteinCryoprotectionEggsEmbryosXenopus laevis

Identifiers

PMID35014670
PMCPMC8920033
OpenAlexW4205352506

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.