ArticleEuropean archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry2022
Label-free quantitative proteomics reveals molecular correlates of altered biomechanical properties in molar incisor hypomineralization (MIH): an in vitro study.
Article in European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.
- Respiratory problems in early childhood and molar-incisor hypomineralization: systematic review and meta-analysis.Odontology · 2026Pooled it
- Comparative label-free quantitative proteomics of hypomineralised second primary molars (HSPM) and molar incisor hypomineralisation (MIH) reveals divergent enamel protein signatures underpinning distinct pathogenic mechanisms.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2026Article
- Comparative evaluation of remineralization agents in severe enamel hypomineralization using a porcine enamel model.BMC oral health · 2026Article
- Micro-Raman Spectroscopy Reveals Compositional Alterations in Molar Incisor Hypomineralization and Enamel Adjacent to Demarcated Opacities.Calcified tissue international · 2026Article
- Reduced Dietary Protein Induces Changes in the Dental Proteome.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2026Article
- Article
- A proteomics approach to understand the hypomineralisation of second primary molars (HSPM). In-vitro study.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2025Article
- A scoping review of proteomic alterations in molar-incisor hypomineralisation: insights from enamel and salivary composition.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2025Article
- Analysis of MMP-8, MMP-20, and TGF-β1 in molar-incisor hypomineralization and assessment of periodontal health.BMC oral health · 2025Observational
- Differential Expression of Hard Tissue Proteins in Hypomineralized Second Primary Molars in Comparison to Normal Teeth.Clinical and experimental dental research · 2025Article
- Storage conditions differentially alter the human tooth enamel proteome.Frontiers in dental medicine · 2025Article
- Proteomic profiling of human dental enamel affected by molar incisor hypomineralisation of different clinical severity grades: an in vitro study.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2024Article
- Implication of serum and salivary albumin tests in the recent oral health related epidemiological studies: A narrative review.The Saudi dental journal · 2024Review
- Matrix Metalloproteinases in the Periodontium-Vital in Tissue Turnover and Unfortunate in Periodontitis.International journal of molecular sciences · 2024Review
- Computerised assessment-a novel approach for calculation of percentage of hypomineralized lesion on incisors and its correlation with aesthetic concern.Journal of oral biology and craniofacial researchArticle
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeMolar-incisor hypomineralization (MIH) is a qualitative developmental defect of enamel that affects first permanent molars with or without affecting permanent incisors. We aimed to carry out a quantitative proteomics-based study to compare and evaluate proteins in sound and MIH-affected enamel. MATERIALS AND
methodsTen blocks each of the MIH-affected enamel and sound enamel were processed and prepared for LC-MS/MS analysis. Label-free quantitation was carried out to evaluate the differentially expressed proteins in the two groups of samples.
resultsA significant increase in the number of proteins in MIH-affected enamel (50.3 ± 29.6) was observed compared to the sound enamel (21.4 ± 3.2). While proteins like collagens, α1-anti-trypsin, kallikrein-4 (KLK4), matrix metalloprotease-20 (MMP-20), alpha-2-macroglobulin, and alpha-2-HS-glycoprotein were upregulated in sound enamel, there was over-expression of albumin, calcium-binding proteins, anti-thrombin III, and dentin sialophosphoprotein (DSPP), along with proteins implicated in stress response and inflammatory processes in MIH.
conclusionWe propose that altered biomechanical properties of the enamel in MIH samples arise due to (i) down-regulation of proteins contributing to collagen biosynthesis and fibril formation; (ii) an overall imbalance in required levels of proteases (KLK4 and MMP-20) and anti-proteases (anti-thrombin-III which inhibits KLK-4), essential for optimal mineralization; (iii) very low levels of alpha-2-macroglobulin with important consequences in enamel mineralization and amelogenesis; and (iv) increased albumin in MIH, preventing proper growth of hydroxyapatite crystals. Increased inflammatory component was also seen in MIH; however, whether inflammation is a cause or consequence of the poor mineralization process needs to be assessed.
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