ArticleJournal of assisted reproduction and genetics2022
Comparison of day 5 blastocyst with day 6 blastocyst: Evidence from NGS-based PGT-A results.
Article in Journal of assisted reproduction and genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Pregnancy and perinatal outcomes after day 5 versus day 6 blastocyst-stage embryo transfer: A systematic review and meta-analysis.Acta obstetricia et gynecologica Scandinavica · 2026Pooled it
- Embryological and clinical predictors of implantation and live birth in 3000 single euploid embryo transfer cycles: a retrospective cohort study.Archives of gynecology and obstetrics · 2026Article
- PGT-A versus expectant management for fertile patients with recurrent pregnancy loss and prior aneuploidy: a dual-center, real-world study of 5-year cumulative pregnancy outcomes.Frontiers in endocrinology · 2026Observational
- Analysis of related factors of biochemical pregnancy loss in single blastocyst transfer in the hormone replacement protocol frozen-thawed cycle.BMC pregnancy and childbirth · 2025Article
- Does Developmental Timing Matter? Comparative Analysis of Day 5 and Day 6 Euploid Blastocyst Transfers in Recurrent Implantation Failure Patients.Biomedicines · 2025Article
- ChromInst: a multicentre evaluation of robustness in aneuploidy and structural rearrangement testing.Journal of translational medicine · 2025Article
- Impact of euploid blastocyst developmental stage and morphological grading on pregnancy outcomes in young recurrent pregnancy loss patients: association with parental chromosomal status.Frontiers in endocrinology · 2025Article
- Effect of day 5 or 6 blastocyst embryo transfer on pregnancy outcomes in euthyroid women undergoing IVF: A single centre retrospective cohort.JBRA assisted reproduction · 2024Article
- Differences in preimplantation blastocyst chromosomal aberrations between polycystic ovary syndrome women and controls: a multi-center retrospective cohort study.Journal of assisted reproduction and genetics · 2024Article
- Machine learning in time-lapse imaging to differentiate embryos from young vs old mice†.Biology of reproduction · 2024Article
- The Impact of Embryo Quality on Pregnancy Outcomes in Single Day 5 versus Day 6 Euploid Blastocyst Transfer: A Retrospective Cohort Study.International journal of fertility & sterility · 2024Article
- Should non-invasive prenatal testing be recommended for patients who achieve pregnancy with PGT?BMC pregnancy and childbirth · 2024Article
- Correlation between controlled ovarian stimulation protocols and euploid blastocyst rate in pre-implantation genetic testing for aneuploidy cycles.Reproductive biology and endocrinology : RB&E · 2023Article
- Pregnancy Outcomes for Day 5 Versus Day 6 Single Frozen-thawed Blastocyst Transfer with Different Qualities of Embryos: A Large Matched-cohort Study.Current medical science · 2023Article
- Morphometric assessment of blastocysts: relationship with the ongoing pregnancy rate.F&S reports · 2023Article
- Delayed blastocyst development is influenced by the level of progesterone on the day of trigger.Journal of assisted reproduction and genetics · 2023Article
- Do chromosomal inversion carriers really need preimplantation genetic testing?Journal of assisted reproduction and genetics · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeAneuploidy is one genetic factor leading to the failure of embryo implantation. This study aimed to investigate the relationship between the day of embryo blastulation and the ploidy status of embryo, to aid in selecting embryos with the most likelihood of of being euploid in a noninvasive way.
methodsThis retrospective study recruited women undergoing preimplantation genetic testing (PGT) for aneuploidy (PGT-A) with trophectoderm biopsy from January 2019 to December 2020. The ploidy status of embryos was determined by next-generation sequencing (NGS).
resultsAltogether, 2531 blastocysts from 839 PGT-A cycles were evaluated. The euploid rate of day 5 blastocysts was significantly higher than that of day 6 blastocysts, either from the same ovarian stimulation (OS) cycles (49.9% vs 35.7%, P < 0.001) or from different OS cycles (48.2% vs 27.8%, P < 0.001). This effect of increasing time to embryo blastulation significantly reducing the prevalence of euploidy was not seen in women at age 38 or older. However, after single euploid embryo transfer, the clinical outcomes of day 5 blastocysts were comparable to those of day 6 blastocysts.
conclusionsIn non-PGT cycles, our data support the selection of day 5 blastocysts for transfer over day 6 blastocysts. Further, women with poor ovarian reserve incline to obtain only day 6 blastocysts, and PGT-A is valuable for identifying the ploidy status of embryos, especially for those with advanced age. However, the stage of blastocysts will not affect the clinical outcome after transfer when they are identified as euploid.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.