ArticleAnalytical and bioanalytical chemistry2022
Structural characterization of phospholipids and sphingolipids by in-source fragmentation MALDI/TOF mass spectrometry.
Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- Microbial biopriming and germination cooperatively remodel brown lentil seeds (Lens culinaris L.) metabolome and antidiabetic functionality.NPJ science of food · 2026Article
- Multiplexed Targeted Spatial Mass Spectrometry Imaging Assays to monitor lipids and NADResearch square · 2025Article
- Structural characterization of sphingomyelins from tissue using electron-induced dissociation.Rapid communications in mass spectrometry : RCM · 2024Article
- Development of a New Binary Matrix for the Comprehensive Analysis of Lipids and Pigments in Micro- and Macroalgae Using MALDI-ToF/ToF Mass Spectrometry.International journal of molecular sciences · 2024Article
- The DDHD2-STXBP1 interaction mediates long-term memory via generation of saturated free fatty acids.The EMBO journal · 2024Article
- Mass Spectrometry-based Single-Cell Lipidomics: Advancements, Challenges, and the Path Forward.Trends in analytical chemistry : TRAC · 2023Article
- rMSIfragment: improving MALDI-MSI lipidomics through automated in-source fragment annotation.Journal of cheminformatics · 2023Article
- A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids.Biomolecules · 2023Review
- Lipids and Secretory Vesicle Exocytosis.Advances in neurobiology · 2023Article
- Analysis of triacylglycerol and phospholipid sn-positional isomers by liquid chromatographic and mass spectrometric methodologies.Mass spectrometry reviewsReview
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2 authors.
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Abstract
Phospholipids (PLs) and sphingolipids (SLs) perform critical structural and biological functions in cells. The structure of these lipids, including the stereospecificity and double-bond position of fatty acyl (FA) chains, is critical in decoding lipid biology. In this study, we presented a simple in-source fragmentation (ISF) MALDI/TOF mass spectrometry method that affords complete structural characterization of PL and SL molecules. We analyzed several representative unsaturated lipid species including phosphatidylcholine (PC), plasmalogen PC (pPC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), cardiolipin (CL), sphingomyelin (SM), and ceramide (Cer). Fragment ions reflecting the FA chains at sn-1 and sn-2 position, and those characteristics of the head groups of different PL classes, are readily identified. Specific fragment ions from cleavages of the C-C bond immediately adjacent to the cis C=C double-bond position(s) of FA chains and the trans C=C double bond of the sphingosine constituents allow precise localization of double bonds. The identities of the exemplary product ions from vinylic, allylic, and double-bond cleavages were also verified by LIFT-TOF/TOF. Identification of individual PL species in the lipid mixture was also carried out with ISF-MALDI/TOF. Together, this approach provides a simple yet effective method for structural characterization of PLs and SLs without the additional modification on the instrument hardware, and serves as a simple tool for the identification of lipids.
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