Evidence map›Paper›PMID 35013616›Full record

ReviewNature protocols2022

KAS-seq: genome-wide sequencing of single-stranded DNA by N

Ruitu Lyu, Tong Wu, Allen C Zhu, Diana C West-Szymanski, Xiaocheng Weng, Mengjie Chen, Chuan He

Abstract readReview
In one paragraph

Review in Nature protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. A nucleic acid labeling chemistry reveals surface DNA on exosomes.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  6. Article
  7. Review
  8. A nucleic acid labeling chemistry reveals surface DNA on exosomes.bioRxiv : the preprint server for biology · 2025
    Article
  9. Article
  10. Article
  11. Zeaxanthin augments CD8Cell reports. Medicine · 2025
    Article
  12. Tandem ssDNA in neutrophil extracellular traps binds thrombin and regulates immunothrombosis.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  13. Article
  14. Article
  15. Benchmarking DNA large language models on quadruplexes.Computational and structural biotechnology journal · 2025
    Article
  16. Article
  17. Article
  18. Article
  19. G-quadruplex DNA structure is a positive regulator ofProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ruitu Lyu *Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA.
Tong Wu *Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA.ORCID http://orcid.org/0000-0001-9968-9909
Allen C ZhuDepartment of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA.
Diana C West-SzymanskiDepartment of Medicine, The University of Chicago, Chicago, IL, USA.
Xiaocheng WengCollege of Chemistry and Molecular Sciences, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei, China.ORCID http://orcid.org/0000-0002-0605-8495
Mengjie ChenDepartment of Medicine, The University of Chicago, Chicago, IL, USA.
Chuan HeDepartment of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA. chuanhe@uchicago.edu.ORCID http://orcid.org/0000-0003-4319-7424

Funding

VIRAL ONCOLOGY CORE FACILITYP30CA014599 · NCI · UNIVERSITY OF CHICAGO · PI MARK W. LINGEN · 1985 to 2026
$122.1M
Transcriptome-wide base-resolution sequencing of pseudouridine in human normal and AD brain tissues-CEGS Admin supplement 2020-2021RM1HG008935 · NHGRI · UNIVERSITY OF CHICAGO · PI HE, CHUAN · 2016 to 2025
$24.4M
Labeling and sequencing of 5hmC and 5mC in DNA-RenewalR01HG006827 · NHGRI · UNIVERSITY OF CHICAGO · PI HE, CHUAN · 2012 to 2025
$7.2M
Translational models of neurodevelopmental epigenetics and disease using human iPSCsP01NS097206 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI SONG, HONGJUN · 2016 to 2020
$4.3M
The Effects of ERK2-Induced Phosphorylation of METTL3 on N6-methyladenosine (m6A) mRNA Methylation in MelanomaF30CA247175 · NCI · UNIVERSITY OF CHICAGO · PI ZHU, ALLEN C · 2020 to 2023
$192k
Howard Hughes Medical InstituteNCI NIH HHS F30 CA247175NCI NIH HHS P30 CA014599NHGRI NIH HHS R01 HG006827NHGRI NIH HHS RM1 HG008935NINDS NIH HHS P01 NS097206
6 · The paper itself

Abstract

Transcription and its dynamics are crucial for gene expression regulation. However, very few methods can directly read out transcriptional activity with low-input material and high temporal resolution. This protocol describes KAS-seq, a robust and sensitive approach for capturing genome-wide single-stranded DNA (ssDNA) profiles using N

Indexed as

DNA, Single-StrandedAnimalsBiotinylationClick ChemistryHumansMiceSequence Analysis, DNADNA, Single-Stranded

Identifiers

PMID35013616
PMCPMC8923001

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.