ArticleCommunications biology2022
Improved clearing method contributes to deep imaging of plant organs.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- The IKU2/RLK7-MAPK-MINI3 signaling module controls endosperm cellularization timing to determine seed size in Arabidopsis.Journal of integrative plant biology · 2026Article
- A simple cell-cycle control system in Marchantia polymorpha provides a framework for understanding plant cell proliferation.The Plant cell · 2026Article
- Initiation of asexual reproduction by the AP2/ERF gene GEMMIFER in Marchantia polymorpha.Current biology : CB · 2026Article
- Florigen and cytokinin signaling antagonistically regulate FLOWERING LOCUS T-LIKE1 to drive a florigen relay that facilitates inflorescence development in rice.Science advances · 2025Article
- Plants in the expansion era: recent advances in expansion microscopy in plant systems.Journal of experimental botany · 2025Review
- Why "Where" Matters as Much as "How Much": Single-Cell and Spatial Transcriptomics in Plants.International journal of molecular sciences · 2025Review
- A Simple and Fast Optical Clearing Method for Whole-Mount Fluorescence In Situ Hybridization (FISH) Imaging.Journal of biophotonics · 2025Article
- SHOT GLASS, an R2R3-MYB transcription factor, promotes gemma cup and gametangiophore development in Marchantia polymorpha.The New phytologist · 2025Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- Article
- A holistic visualization for quality of Chinese materia medica: Structural and metabolic visualization by magnetic resonance imaging.Journal of pharmaceutical analysis · 2024Review
- Clearing techniques for deeper imaging of plants and plant-microbe interactions.Applied microscopy · 2024Review
- The Function of Florigen in the Vegetative-to-Reproductive Phase Transition in and around the Shoot Apical Meristem.Plant & cell physiology · 2024Review
- An optimized live imaging and growth analysis approach for Arabidopsis Sepals.bioRxiv : the preprint server for biology · 2024Article
- An optimized live imaging and multiple cell layer growth analysis approach using Arabidopsis sepals.Frontiers in plant science · 2024Article
- Biosynthesis of gibberellin-related compounds modulates far-red light responses in the liverwort Marchantia polymorpha.The Plant cell · 2023Article
- Control of Plant Cell Growth and Proliferation by MO25A, a Conserved Major Component of the Mammalian Sterile 20-Like Kinase Pathway.Plant & cell physiology · 2023Article
- Sachihiro Matsunaga: FISHing the nuclear architecture of plant cells.The Journal of cell biology · 2022Article
- Comparing the efficiency of six clearing methods in developing seeds of Arabidopsis thaliana.Plant reproduction · 2022Article
- The renaissance and enlightenment of Marchantia as a model system.The Plant cell · 2022Article
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
Tissue clearing methods are increasingly essential for the microscopic observation of internal tissues of thick biological organs. We previously developed TOMEI, a clearing method for plant tissues; however, it could not entirely remove chlorophylls nor reduce the fluorescent signal of fluorescent proteins. Here, we developed an improved TOMEI method (iTOMEI) to overcome these limitations. First, a caprylyl sulfobetaine was determined to efficiently remove chlorophylls from Arabidopsis thaliana seedlings without GFP quenching. Next, a weak alkaline solution restored GFP fluorescence, which was mainly lost during fixation, and an iohexol solution with a high refractive index increased sample transparency. These procedures were integrated to form iTOMEI. iTOMEI enables the detection of much brighter fluorescence than previous methods in tissues of A. thaliana, Oryza sativa, and Marchantia polymorpha. Moreover, a mouse brain was also efficiently cleared by the iTOMEI-Brain method within 48 h, and strong fluorescent signals were detected in the cleared brain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.