ArticleScientific reports2022
Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 11 citations in OpenAlex.
- Development of plastome-based HRM markers for the authentication of 12 major medicinal herbs in the Apiaceae family.Scientific reports · 2026Article
- Detection of Certain Berries in Difficult Samples by Singleplex and Multiplex Real-Time PCR-HRM: A Case Study of Pitfalls.Methods and protocols · 2026Article
- Isolation and characterization of lyticFrontiers in cellular and infection microbiology · 2026Article
- High-Resolution Melting PCR as a Fast and Simple Molecular Biology-Based Method for the Identification of HypervirulentMicroorganisms · 2024Article
- Distinguishing fanged frogs (Limnonectes) species (Amphibia: Anura: Dicroglossidae), from Thailand using high resolution melting analysis.Scientific reports · 2023Article
- Molecular Mechanisms ofInternational journal of molecular sciences · 2023Review
- Comparative Study on Tuberculosis Drug Resistance and Molecular Detection Methods Among Different Mycobacterium Tuberculosis Lineages.Infection and drug resistance · 2023Article
- Characterizations of novel broad-spectrum lytic bacteriophagesFrontiers in microbiology · 2023Article
- Molecular diagnosis of bovine genital campylobacteriosis using high-resolution melting analysis.Frontiers in microbiology · 2022Article
- Development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulentFrontiers in cellular and infection microbiology · 2022Article
- Multiplex high-resolution melting assay for simultaneous detection of five key bacterial pathogens in urinary tract infections: A pilot study.Frontiers in microbiology · 2022Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shigella species, a group of intracellular foodborne pathogens, are the main causes of bacillary dysentery and shigellosis in humans worldwide. It is essential to determine the species of Shigella in outbreaks and food safety surveillance systems. The available immunological and molecular methods for identifying Shigella species are relatively complicated, expensive and time-consuming. High resolution melting (HRM) assay is a rapid, cost-effective, and easy to perform PCR-based method that has recently been used for the differentiation of bacterial species. In this study, we designed and developed a PCR-HRM assay targeting rrsA gene to distinguish four species of 49 Shigella isolates from clinical and food samples and evaluated the sensitivity and specificity of the assay. The assay demonstrated a good analytical sensitivity with 0.01-0.1 ng of input DNA template and an analytical specificity of 100% to differentiate the Shigella species. The PCR-HRM assay also was able to identify the species of all 49 Shigella isolates from clinical and food samples correctly. Consequently, this rapid and user-friendly method demonstrated good sensitivity and specificity to differentiate species of the Shigella isolates from naturally contaminated samples and has the potential to be implemented in public health and food safety surveillance systems.
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