ArticleScientific reports2022
Analysis of the cells isolated from epithelial cell rests of Malassez through single-cell limiting dilution.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07587567 (Determination of Risk Factors for Eruption Disorders in the Primary and Permanent Dentition), which is not on this map. Cited by 4 papers.
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Determination of Risk Factors for Eruption Disorders in the Primary and Permanent Dentition
Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- Transcriptomic response to compressive force simulating orthodontic tooth movement in cultured porcine epithelial cell rests of Malassez.Odontology · 2026Article
- Effect of conditioned medium derived from a clone cell of epithelial rests of Malassez on enamel crystallization in tooth germs.Medical molecular morphology · 2026Article
- Permanent first molar eruption failure in children: leading signs for early diagnosis.Progress in orthodontics · 2025Observational
- Proteome alterations during clonal isolation of established human pancreatic cancer cell lines.Cellular and molecular life sciences : CMLS · 2022Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The epithelial cell rests of Malassez (ERM) are essential in preventing ankylosis between the alveolar bone and the tooth (dentoalveolar ankylosis). Despite extensive research, the mechanism by which ERM cells suppress ankylosis remains uncertain; perhaps its varied population is to reason. Therefore, in this study, eighteen unique clones of ERM (CRUDE) were isolated using the single-cell limiting dilution and designated as ERM 1-18. qRT-PCR, ELISA, and western blot analyses revealed that ERM-2 and -3 had the highest and lowest amelogenin expression, respectively. Mineralization of human periodontal ligament fibroblasts (HPDLF) was reduced in vitro co-culture with CRUDE ERM, ERM-2, and -3 cells, but recovered when an anti-amelogenin antibody was introduced. Transplanted rat molars grown in ERM-2 cell supernatants produced substantially less bone than those cultured in other cell supernatants; inhibition was rescued when an anti-amelogenin antibody was added to the supernatants. Anti-Osterix antibody staining was used to confirm the development of new bones. In addition, next-generation sequencing (NGS) data were analysed to discover genes related to the distinct roles of CRUDE ERM, ERM-2, and ERM-3. According to this study, amelogenin produced by ERM cells helps to prevent dentoalveolar ankylosis and maintain periodontal ligament (PDL) space, depending on their clonal diversity.
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