ArticleScientific reports2022
High-throughput formation and image-based analysis of basal-in mammary organoids in 384-well plates.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
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- The Spheroid Light Microscopy Image Atlas for morphometrical analysis of three-dimensional cell cultures.Scientific data · 2025Article
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- Harnessing the power of artificial intelligence for human living organoid research.Bioactive materials · 2024Review
- Dental pulp stem cells-derived cannabidiol-treated organoid-like microspheroids show robust osteogenic potential via upregulation of WNT6.Communications biology · 2024Article
- 3D Culture Analysis of Cancer Cell Adherence toTissue engineering. Part C, Methods · 2024Article
- Development of robust antiviral assays using relevant apical-out human airway organoids.bioRxiv : the preprint server for biology · 2024Article
- Modeling of the brain-lung axis using organoids in traumatic brain injury: an updated review.Cell & bioscience · 2024Review
- OctoShaker: A versatile robotic biomechanical agitator for cellular and organoid research.The Review of scientific instruments · 2023Article
- A microwell platform for high-throughput longitudinal phenotyping and selective retrieval of organoids.Cell systems · 2023Article
- Optimal sparsity allows reliable system-aware restoration of fluorescence microscopy images.Science advances · 2023Article
- Engineered biomaterials to guide spheroid formation, function, and fabrication into 3D tissue constructs.Acta biomaterialia · 2023Review
- Triple-negative breast cancer cells invade adipocyte/preadipocyte-encapsulating geometrically inverted mammary organoids.Integrative biology : quantitative biosciences from nano to macro · 2023Article
- Biomimetic Gland Models with Engineered Stratagems.Research (Washington, D.C.) · 2023Review
- 3D super-resolution live-cell imaging with radial symmetry and Fourier light-field microscopy.Biomedical optics express · 2022Article
- Extended longevity geometrically-inverted proximal tubule organoids.Biomaterials · 2022Article
- Better In Vitro Tools for ExploringLife (Basel, Switzerland) · 2022Article
- Assay establishment and validation of a high-throughput organoid-based drug screening platform.Stem cell research & therapy · 2022Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
This manuscript describes a new method for forming basal-in MCF10A organoids using commercial 384-well ultra-low attachment (ULA) microplates and the development of associated live-cell imaging and automated analysis protocols. The use of a commercial 384-well ULA platform makes this method more broadly accessible than previously reported hanging drop systems and enables in-incubator automated imaging. Therefore, time points can be captured on a more frequent basis to improve tracking of early organoid formation and growth. However, one major challenge of live-cell imaging in multi-well plates is the rapid accumulation of large numbers of images. In this paper, an automated MATLAB script to handle the increased image load is developed. This analysis protocol utilizes morphological image processing to identify cellular structures within each image and quantify their circularity and size. Using this script, time-lapse images of aggregating and non-aggregating culture conditions are analyzed to profile early changes in size and circularity. Moreover, this high-throughput platform is applied to widely screen concentration combinations of Matrigel and epidermal growth factor (EGF) or heparin-binding EGF-like growth factor (HB-EGF) for their impact on organoid formation. These results can serve as a practical resource, guiding future research with basal-in MCF10A organoids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.