Evidence map›Paper›PMID 35013299›Full record

ArticleNature communications2022

Single cell transcriptomic landscape of diabetic foot ulcers.

Georgios Theocharidis, Beena E Thomas, Debasree Sarkar, Hope L Mumme, William J R Pilcher, Bhakti Dwivedi, Teresa Sandoval-Schaefer, Ruxandra F Sîrbulescu, Antonios Kafanas, Ikram Mezghani and 9 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 254 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
254citing papers in PubMed, 1 pooled it
50.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

254 citing papers in PubMed, 1 synthesis or guideline pooled it, 366 citations in OpenAlex.

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194 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 5 institutions in 2 countries.

Georgios Theocharidis *The Rongxiang Xu, MD, Center for Regenerative Therapeutics and Joslin-Beth Israel Deaconess Foot Center, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Beena E Thomas *Aflac Cancer and Blood Disorders Center, Children Healthcare of Atlanta, Department of Pediatrics and Biomedical Informatics, Emory University, Atlanta, GA, USA.
Debasree Sarkar *Aflac Cancer and Blood Disorders Center, Children Healthcare of Atlanta, Department of Pediatrics and Biomedical Informatics, Emory University, Atlanta, GA, USA.ORCID http://orcid.org/0000-0002-3228-9131
Hope L MummeAflac Cancer and Blood Disorders Center, Children Healthcare of Atlanta, Department of Pediatrics and Biomedical Informatics, Emory University, Atlanta, GA, USA.ORCID http://orcid.org/0000-0002-4171-1736
William J R PilcherAflac Cancer and Blood Disorders Center, Children Healthcare of Atlanta, Department of Pediatrics and Biomedical Informatics, Emory University, Atlanta, GA, USA.
Bhakti DwivediWinship Cancer Institute, Emory University, Atlanta, GA, USA.
Teresa Sandoval-SchaeferMolecular, Cellular and Developmental Biology, Yale University, New Haven, CT, USA.
Ruxandra F SîrbulescuVaccine and Immunotherapy Center, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0001-7905-1713
Antonios KafanasLincoln County Hospital, Northern Lincolnshire and Goole NHS Foundation Trust, Scunthorpe, UK.
Ikram MezghaniThe Rongxiang Xu, MD, Center for Regenerative Therapeutics and Joslin-Beth Israel Deaconess Foot Center, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Peng WangThe Rongxiang Xu, MD, Center for Regenerative Therapeutics and Joslin-Beth Israel Deaconess Foot Center, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Antonio LobaoThe Rongxiang Xu, MD, Center for Regenerative Therapeutics and Joslin-Beth Israel Deaconess Foot Center, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Ioannis S VlachosDepartment of Pathology, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, MA, USA.
Biraja DashYale Plastic and Reconstructive Surgery-Wound Center, Yale School of Medicine, New Haven, CT, USA.
Henry C HsiaYale Plastic and Reconstructive Surgery-Wound Center, Yale School of Medicine, New Haven, CT, USA.
Valerie HorsleyMolecular, Cellular and Developmental Biology, Yale University, New Haven, CT, USA.ORCID http://orcid.org/0000-0002-1254-5839
Swati S BhasinAflac Cancer and Blood Disorders Center, Children Healthcare of Atlanta, Department of Pediatrics and Biomedical Informatics, Emory University, Atlanta, GA, USA.ORCID http://orcid.org/0000-0001-8661-1459
Aristidis VevesThe Rongxiang Xu, MD, Center for Regenerative Therapeutics and Joslin-Beth Israel Deaconess Foot Center, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA. aveves@bidmc.harvard.edu.ORCID http://orcid.org/0000-0002-3901-4405
Manoj BhasinAflac Cancer and Blood Disorders Center, Children Healthcare of Atlanta, Department of Pediatrics and Biomedical Informatics, Emory University, Atlanta, GA, USA. manoj.bhasin@emory.edu.
Emory University · USBeth Israel Deaconess Medical Center · USYale University · USHarvard University · USLincoln County Hospital · GB

Funding

Yale Clinical and Translational Science Award (U Component)UL1TR001863 · NCATS · YALE UNIVERSITY · PI John H. Krystal, LUCILA OHNO-MACHADO · 2016 to 2026
$102.9M
Coordinating Unit for DiaCompU24DK115255 · NIDDK · AUGUSTA UNIVERSITY · PI MCINDOE, RICHARD A. · 2017 to 2020
$11.6M
NCATS NIH HHS UL1 TR001863NIDDK NIH HHS U24 DK115255
6 · The paper itself

Abstract

Diabetic foot ulceration (DFU) is a devastating complication of diabetes whose pathogenesis remains incompletely understood. Here, we profile 174,962 single cells from the foot, forearm, and peripheral blood mononuclear cells using single-cell RNA sequencing. Our analysis shows enrichment of a unique population of fibroblasts overexpressing MMP1, MMP3, MMP11, HIF1A, CHI3L1, and TNFAIP6 and increased M1 macrophage polarization in the DFU patients with healing wounds. Further, analysis of spatially separated samples from the same patient and spatial transcriptomics reveal preferential localization of these healing associated fibroblasts toward the wound bed as compared to the wound edge or unwounded skin. Spatial transcriptomics also validates our findings of higher abundance of M1 macrophages in healers and M2 macrophages in non-healers. Our analysis provides deep insights into the wound healing microenvironment, identifying cell types that could be critical in promoting DFU healing, and may inform novel therapeutic approaches for DFU treatment.

Indexed as

TranscriptomeBiomarkersCell Adhesion MoleculesChitinase-3-Like Protein 1Diabetes MellitusDiabetic FootEndothelial CellsExome SequencingFibroblastsGene Expression RegulationHigh-Throughput Nucleotide SequencingHumansHypoxia-Inducible Factor 1, alpha SubunitKeratinocytesLeukocytesMacrophagesBiomarkersCell Adhesion MoleculesCHI3L1 protein, humanChitinase-3-Like Protein 1HIF1A protein, humanHypoxia-Inducible Factor 1, alpha SubunitMatrix Metalloproteinase 1Matrix Metalloproteinase 11Matrix Metalloproteinase 3MMP11 protein, humanMMP1 protein, humanMMP3 protein, humanTNFAIP6 protein, human

Identifiers

PMID35013299
PMCPMC8748704
OpenAlexW4226016727

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.