Evidence map›Paper›PMID 35013146›Full record

ArticleNature communications2022

Single-cell analysis of human primary prostate cancer reveals the heterogeneity of tumor-associated epithelial cell states.

Hanbing Song, Hannah N W Weinstein, Paul Allegakoen, Marc H Wadsworth, Jamie Xie, Heiko Yang, Ethan A Castro, Kevin L Lu, Bradley A Stohr, Felix Y Feng and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 203 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
203citing papers in PubMed, 1 pooled it
25.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

203 citing papers in PubMed, 1 synthesis or guideline pooled it, 250 citations in OpenAlex.

  1. Pooled it
  2. Cambridge Neoadjuvant Cancer of the Prostate (CANCAP03): A Window Study into the Effects of Olaparib ± Degarelix in Primary Prostate Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2025
    Trial
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  8. Article
  9. Article
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  14. Advances in spatial omics for the analysis of prostate cancer.Analytical and bioanalytical chemistry · 2026
    Review
  15. Review
  16. Review
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  18. Article
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  20. Review

143 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Hanbing SongDivision of Hematology/Oncology, Department of Medicine, University of California, San Francisco, San Francisco, CA, 94143, USA.ORCID http://orcid.org/0000-0002-2164-8813
Hannah N W Weinstein *Division of Hematology/Oncology, Department of Medicine, University of California, San Francisco, San Francisco, CA, 94143, USA.ORCID http://orcid.org/0000-0001-8409-4654
Paul Allegakoen *Division of Hematology/Oncology, Department of Medicine, University of California, San Francisco, San Francisco, CA, 94143, USA.
Marc H Wadsworth *The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard University, Cambridge, MA, 02139, USA.
Jamie XieDivision of Hematology/Oncology, Department of Medicine, University of California, San Francisco, San Francisco, CA, 94143, USA.
Heiko YangHelen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA, 94143, USA.
Ethan A CastroDivision of Hematology/Oncology, Department of Medicine, University of California, San Francisco, San Francisco, CA, 94143, USA.
Kevin L LuDepartment of Pathology, University of California, San Francisco, San Francisco, CA, 94143, USA.
Bradley A StohrDepartment of Pathology, University of California, San Francisco, San Francisco, CA, 94143, USA.
Felix Y FengHelen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA, 94143, USA.ORCID http://orcid.org/0000-0002-0963-7687
Peter R CarrollHelen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA, 94143, USA.
Bruce WangDivision of Gastroenterology, Department of Medicine, University of California, San Francisco, CA, 94143, USA.ORCID http://orcid.org/0000-0003-3486-5494
Matthew R CooperbergHelen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA, 94143, USA.
Alex K ShalekThe Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard University, Cambridge, MA, 02139, USA.ORCID http://orcid.org/0000-0001-5670-8778
Franklin W HuangDivision of Hematology/Oncology, Department of Medicine, University of California, San Francisco, San Francisco, CA, 94143, USA. Franklin.Huang@ucsf.edu.ORCID http://orcid.org/0000-0001-5447-0436
University of California, San Francisco · USBroad Institute · USSan Francisco VA Medical Center · US

Funding

Precision Interception of Aggressive Prostate Cancer in African American MenP20CA233255 · NCI · DANA-FARBER CANCER INST · PI REBBECK, TIMOTHY R · 2018 to 2020
$3.2M
NCI NIH HHS P20 CA233255
6 · The paper itself

Abstract

Prostate cancer is the second most common malignancy in men worldwide and consists of a mixture of tumor and non-tumor cell types. To characterize the prostate cancer tumor microenvironment, we perform single-cell RNA-sequencing on prostate biopsies, prostatectomy specimens, and patient-derived organoids from localized prostate cancer patients. We uncover heterogeneous cellular states in prostate epithelial cells marked by high androgen signaling states that are enriched in prostate cancer and identify a population of tumor-associated club cells that may be associated with prostate carcinogenesis. ERG-negative tumor cells, compared to ERG-positive cells, demonstrate shared heterogeneity with surrounding luminal epithelial cells and appear to give rise to common tumor microenvironment responses. Finally, we show that prostate epithelial organoids harbor tumor-associated epithelial cell states and are enriched with distinct cell types and states from their parent tissues. Our results provide diagnostically relevant insights and advance our understanding of the cellular states associated with prostate carcinogenesis.

Indexed as

CarcinogenesisCell LineageEpithelial CellsGene Expression ProfilingGene Expression Regulation, NeoplasticGene OntologyGenetic HeterogeneityHumansMaleMolecular Sequence AnnotationNeoplasm ProteinsOrganoidsPrimary Cell CultureProstateProstatectomyProstatic NeoplasmsERG protein, humanNeoplasm ProteinsTranscriptional Regulator ERG

Identifiers

PMID35013146
PMCPMC8748675
OpenAlexW4220765144

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.