Evidence map›Paper›PMID 35013075›Full record

ArticleThe Journal of craniofacial surgery

Effect of Stromal Vascular Fractions on Angiogenesis of Injected Diced Cartilage.

Han Yin, Cong Xie, Li Li, Li Zeng, Zi-Wen Zhu, Nian Chen, Hong-Ju Xie, Jun-Lin Liao

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In one paragraph

Article in The Journal of craniofacial surgery. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Han YinDepartments of Medical Cosmetology, The First Affiliated Hospital, University of South China, Hengyang.
Cong XieDepartments of Medical Cosmetology, The First Affiliated Hospital, University of South China, Hengyang.
Li LiDepartments of Medical Cosmetology, The First Affiliated Hospital, University of South China, Hengyang.
Li ZengDepartments of Medical Cosmetology, The First Affiliated Hospital, University of South China, Hengyang.
Zi-Wen ZhuDepartments of Medical Cosmetology, The First Affiliated Hospital, University of South China, Hengyang.
Nian ChenDepartments of Medical Cosmetology, The First Affiliated Hospital, University of South China, Hengyang.
Hong-Ju XieDepartments of Plastic Surgery, The Second Affiliated Hospital of Hainan Medical University, Haikou.
Jun-Lin LiaoDepartments of Medical Cosmetology, The First Affiliated Hospital, University of South China, Hengyang.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThis study explored the effect of adipose-derived stromal vascular fractions (SVFs) on angiogenesis in injected autologous diced cartilage.

methodsStromal vascular fractions were extracted by enzymatic digestion. Cartilage grafts were harvested from 1 side of the auricular cartilage of New Zealand rabbit and then diced to a size of 1.0 mm3. The grafts were divided into 2 groups. The control group was diced cartilage mixed with culture medium, and the experimental group was diced cartilage mixed with SVFs. The 2 groups of composite grafts were subcutaneously implanted on both sides of the back of each rabbit. After 4, 12 and 24 weeks, the tissue structure, number of blood vessels, and angiogenic factors in the grafts were observed.

resultsThe SVFs conformed to the current standard of the biological evaluation. Under an inverted microscope, the number of layers of chondrocytes in the experimental group was higher than that in the control group at 4 weeks. A small number of inflammatory cells and blood vessels were observed around the cartilage grafts. At 12 and 24 weeks, the volume of tissue was increased gradually by general observation. And a large number of chondrocytes were observed microscopically, whereas the number of inflammatory cells decreased. And meanwhile additional new blood vessels were observed. Immunohistochemical analysis of CD31 showed that the number of capillaries in the control group was significantly lower than that in the experimental group at 4, 12 and 24 weeks. Further, the expression of Hypoxia inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) mRNA and protein were measured by RT-PCR and Western bloting, respectively. The results showed that the mRNA expression of VEGF and HIF-1α in the experimental group was increased. The mRNA level remained higher than that of the control group at 24 weeks (P < 0.05). And the relative expression levels of VEGF and HIF-1α protein in the experimental group were higher than those in the control group at 4, 12 and 24 weeks (P < 0.05).

conclusionAutologous diced cartilage mixed with adipose-derived SVFs can promote angiogenesis when transplanted by injection. Further research showed that SVFs could increase the expression levels of VEGF and HIF-1α in the grafts, which may be part of the mechanism that SVFs promoted the angiogenesis of diced cartilage.

Indexed as

Dental ImplantsVascular Endothelial Growth Factor AAnimalsEar CartilageHumansRabbitsRNA, MessengerStromal Vascular FractionDental ImplantsRNA, MessengerVascular Endothelial Growth Factor A

Identifiers

PMID35013075
PMCPMC8865209

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.