Evidence map›Paper›PMID 35012910›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2021

[Effects of lentivirus-mediated RNA interference of HIF-1α and PTEN on oxygen-glucose deprivation injury in primary cultured rat neurons].

J Tang, Y Yang, J Chen, T Li, Y Dai

Open access · greenAbstract read
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In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

J TangDepartment of Child Health Care, Children's Hospital of Chongqing Medical University//National Clinical Research Center for Child Health and Disorders//Ministry of Education Key Laboratory of Child Development and Disorders//Chongqing Key Laboratory of Child Health and Nutrition, Chongqing 40014, China.
Y YangDepartment of Child Health Care, Children's Hospital of Chongqing Medical University//National Clinical Research Center for Child Health and Disorders//Ministry of Education Key Laboratory of Child Development and Disorders//Chongqing Key Laboratory of Child Health and Nutrition, Chongqing 40014, China.
J ChenDepartment of Child Health Care, Children's Hospital of Chongqing Medical University//National Clinical Research Center for Child Health and Disorders//Ministry of Education Key Laboratory of Child Development and Disorders//Chongqing Key Laboratory of Child Health and Nutrition, Chongqing 40014, China.
T LiDepartment of Child Health Care, Children's Hospital of Chongqing Medical University//National Clinical Research Center for Child Health and Disorders//Ministry of Education Key Laboratory of Child Development and Disorders//Chongqing Key Laboratory of Child Health and Nutrition, Chongqing 40014, China.
Y DaiDepartment of Child Health Care, Children's Hospital of Chongqing Medical University//National Clinical Research Center for Child Health and Disorders//Ministry of Education Key Laboratory of Child Development and Disorders//Chongqing Key Laboratory of Child Health and Nutrition, Chongqing 40014, China.
Children's Hospital of Chongqing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo observe the effects of lentivirus-mediated RNA interference (RNAi) of hypoxia-inducible factor 1α (HIF-1α) and phosphatase and tensin homolog on chromosome ten (PTEN) on oxygen-glucose deprivation (OGD) injury in primary cultured rat neurons.

methodsPrimary cultures of neonatal SD rat neurons were infected by lentiviral vectors carrying short hairpin RNA (shRNA) targeting HIF-1α or PTEN followed 4 days later by hypoxic exposure, and the control neurons were infected with the empty virus only with or without subsequent hypoxic exposure. Twenty-four hours after hypoxia, the interference efficiency was assessed with qRT-PCR, and lactate dehydrogenase (LDH) assay and AnnexinV-FITC/ PI assay were performed to detect neuronal damage and apoptosis. The expressions of the related proteins were determined with Western blotting.

resultsLentivirus-mediated RNAi effectively silenced the mRNA expression of the target genes. HIF-1α silencing obviously aggravated the hypoxia-induced damage and apoptosis of the neurons, enhanced the expression of PTEN protein and significantly lowered the expressions of p-PTEN, p-AKT, NR2A and VEGFa (

conclusionAn up-regulated expression of HIF-1α causes down-regulation of PTEN expression to protect primary cultured rat neurons against OGD injury.

Indexed as

Cell HypoxiaLentivirusNeuronsPTEN PhosphohydrolaseAnimalsCells, CulturedGlucoseHypoxia-Inducible Factor 1, alpha SubunitOxygenRatsRats, Sprague-DawleyRNA InterferenceVascular Endothelial Growth Factor AGlucoseHif1a protein, ratHypoxia-Inducible Factor 1, alpha SubunitOxygenPTEN PhosphohydrolasePten protein, ratVascular Endothelial Growth Factor Ahypoxiahypoxia-inducible factor-1αPTEN-PI3K/AKT signal pathRNA Interference

Identifiers

PMID35012910
PMCPMC8752430
OpenAlexW4205290827

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.