ArticleGenome biology2022
Knockout of circRNAs by base editing back-splice sites of circularized exons.
Article in Genome biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- CIRCpedia v3: an interactive database for circular RNA characterization and functional exploration.Nucleic acids research · 2026Article
- Review
- Review
- Engineering back-splicing-like sites for enhanced linear RNA expression.Applied microbiology and biotechnology · 2025Article
- Circular RNA Formation and Degradation Are Not Directed by Universal Pathways.International journal of molecular sciences · 2025Review
- Formation of Circular RNAs.Advances in experimental medicine and biology · 2025Review
- Oxidative Stress-Associated Alteration of circRNA and Their ceRNA Network in Differentiating Neuroblasts.International journal of molecular sciences · 2024Article
- A CRISPR/RfxCas13d-mediated strategy for efficient RNA knockdown in mouse embryonic development.Science China. Life sciences · 2024Article
- Article
- Advanced approaches of the use of circRNAs as a replacement for cancer therapy.Non-coding RNA research · 2024Review
- Techniques for investigating lncRNA transcript functions in neurodevelopment.Molecular psychiatry · 2024Review
- Role of circular RNAs and gut microbiome in gastrointestinal cancers and therapeutic targets.Non-coding RNA research · 2024Review
- In Vivo Tissue-Specific Knockdown of circRNAs Using shRNAs in Drosophila melanogaster.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Base-Editor-Mediated circRNA Knockout by Targeting Predominantly Back-Splice Sites.Methods in molecular biology (Clifton, N.J.) · 2024Article
- New insight into circRNAs: characterization, strategies, and biomedical applications.Experimental hematology & oncology · 2023Review
- Regulatory Functions and Mechanisms of Circular RNAs in Hepatic Stellate Cell Activation and Liver Fibrosis.Cells · 2023Review
- Identification, biogenesis, function, and mechanism of action of circular RNAs in plants.Plant communications · 2023Review
- Circular RNAs in Cardiovascular Diseases: Regulation and Therapeutic Applications.Research (Washington, D.C.) · 2023Review
- Biogenesis and Regulatory Roles of Circular RNAs.Annual review of cell and developmental biology · 2022Review
- A Review and In Silico Analysis of Tissue and Exosomal Circular RNAs: Opportunities and Challenges in Thyroid Cancer.Cancers · 2022Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Many circular RNAs (circRNAs) are produced from back-splicing of exons of precursor mRNAs and are generally co-expressed with cognate linear RNAs. Methods for circRNA-specific knockout are lacking, largely due to sequence overlaps between forms. Here, we use base editors (BEs) for circRNA depletion. By targeting splice sites involved in both back-splicing and canonical splicing, BEs can repress circular and linear RNAs. Targeting sites predominantly for circRNA biogenesis, BEs could efficiently repress the production of circular but not linear RNAs. As hundreds of exons are predominantly back-spliced to produce circRNAs, this provides an efficient method to deplete circRNAs for functional study.
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Registered trials
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