Evidence map›Paper›PMID 35011596›Full record

ReviewCells2021

Fluorescence Microscopy-An Outline of Hardware, Biological Handling, and Fluorophore Considerations.

Shane M Hickey, Ben Ung, Christie Bader, Robert Brooks, Joanna Lazniewska, Ian R D Johnson, Alexandra Sorvina, Jessica Logan, Carmela Martini, Courtney R Moore and 3 more

Open access · goldAbstract readReview
In one paragraph

Review in Cells, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
14.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 113 citations in OpenAlex.

  1. Article
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  11. Spatial organization ofInfection and immunity · 2026
    Article
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  17. Novel Platinum(II) Tetrazine Complex Capable of Live-Cell IEDDA Reaction.Chembiochem : a European journal of chemical biology · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Shane M HickeyClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.ORCID 0000-0002-3063-531X
Ben UngClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.ORCID 0000-0001-7667-3648
Christie BaderClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.
Robert BrooksClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.
Joanna LazniewskaClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.ORCID 0000-0001-5729-4773
Ian R D JohnsonClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.ORCID 0000-0001-7875-3510
Alexandra SorvinaClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.ORCID 0000-0002-5918-3038
Jessica LoganClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.
Carmela MartiniClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.
Courtney R MooreClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.ORCID 0000-0003-3675-4026
Litsa KarageorgosClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.
Martin J SweetmanClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.ORCID 0000-0002-9074-2125
Douglas A BrooksClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.ORCID 0000-0001-9098-3626
University of South Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability; (2) amenability of biological models to fluorescence microscopy; and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology.

Indexed as

Microscopy, FluorescenceAnimalsFluorescent DyesHumansImaging, Three-DimensionalModels, BiologicalOrganellesFluorescent Dyescellular imagingfluorescence microscopyimaging agentsmicroscopy techniques

Identifiers

PMID35011596
PMCPMC8750338
OpenAlexW4200013025

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.