Evidence map›Paper›PMID 35011319›Full record

ArticleMolecules (Basel, Switzerland)2021

Purification, Characterization, and Structural Studies of a Sulfatase from

Caleb R Schlachter, Andrea O'Malley, Linda L Grimes, John J Tomashek, Maksymilian Chruszcz, L Andrew Lee

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Caleb R SchlachterIntegrated Micro-Chromatography Systems, 110 Centrum Drive, Irmo, SC 29063, USA.
Andrea O'MalleyDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA.
Linda L GrimesIntegrated Micro-Chromatography Systems, 110 Centrum Drive, Irmo, SC 29063, USA.
John J TomashekIntegrated Micro-Chromatography Systems, 110 Centrum Drive, Irmo, SC 29063, USA.
Maksymilian ChruszczDepartment of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA.ORCID 0000-0001-7521-5485
L Andrew LeeIntegrated Micro-Chromatography Systems, 110 Centrum Drive, Irmo, SC 29063, USA.
University of South Carolina · US

Funding

University of South Carolina ASPIRE III
6 · The paper itself

Abstract

Sulfatases are ubiquitous enzymes that hydrolyze sulfate from sulfated organic substrates such as carbohydrates, steroids, and flavones. These enzymes can be exploited in the field of biotechnology to analyze sulfated metabolites in humans, such as steroids and drugs of abuse. Because genomic data far outstrip biochemical characterization, the analysis of sulfatases from published sequences can lead to the discovery of new and unique activities advantageous for biotechnological applications. We expressed and characterized a putative sulfatase (PyuS) from the bacterium

Indexed as

Amino Acid SequenceBinding SitesCatalytic DomainChemical FractionationEnzyme StabilityHumansModels, MolecularMolecular StructurePedobacterProtein ConformationProtein MultimerizationRecombinant ProteinsStructure-Activity RelationshipSubstrate SpecificitySulfatasesRecombinant ProteinsSulfatases4-methylumbelliferyl sulfatearylsulfatasecrystallographyformylglycinehydrolasePedobacter yulinensissulfatase

Identifiers

PMID35011319
PMCPMC8746622
OpenAlexW4206017050

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.