Evidence map›Paper›PMID 35009972›Full record

ReviewNanomaterials (Basel, Switzerland)2021

Fluorescent Probes for STED Optical Nanoscopy.

Sejoo Jeong, Jerker Widengren, Jong-Chan Lee

Abstract readReview
In one paragraph

Review in Nanomaterials (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. 3D STED Imaging of Isolated Arabidopsis thaliana Nuclei.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  11. The ATTO 565 Dye and Its Applications in Microscopy.Molecules (Basel, Switzerland) · 2024
    Review
  12. Article
  13. Review
  14. Review
  15. Review
  16. Article
  17. Article
  18. Identifying STEDable BFMolecules (Basel, Switzerland) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sejoo JeongDepartment of New Biology, Daegu Gyeongbuk Institute of Science & Technology, Daegu 42988, Korea.
Jerker WidengrenExperimental Biomolecular Physics, Department of Applied Physics, Royal Institute of Technology (KTH), Stockholm 10691, Sweden.
Jong-Chan LeeDepartment of New Biology, Daegu Gyeongbuk Institute of Science & Technology, Daegu 42988, Korea.ORCID 0000-0003-3073-4657

Funding

DGIST R&D Program of the Ministry of Science and ICT of Korea 20-CoE-BT-04National Research Foundation of Korea 2019K2A9A2A12000363National Research Foundation of Korea 2020R1A4A1019408National Research Foundation of Korea 2021R1I1A2058828Swedish Foundation for International Cooperation in Research and Higher Education 1904-2206/KO2018-7991
6 · The paper itself

Abstract

Progress in developing fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, is inseparable from the advancement in optical fluorescence microscopy. Super-resolution microscopy, or optical nanoscopy, overcame the far-field optical resolution limit, known as Abbe's diffraction limit, by taking advantage of the photophysical properties of fluorescent probes. Therefore, fluorescent probes for super-resolution microscopy should meet the new requirements in the probes' photophysical and photochemical properties. STED optical nanoscopy achieves super-resolution by depleting excited fluorophores at the periphery of an excitation laser beam using a depletion beam with a hollow core. An ideal fluorescent probe for STED nanoscopy must meet specific photophysical and photochemical properties, including high photostability, depletability at the depletion wavelength, low adverse excitability, and biocompatibility. This review introduces the requirements of fluorescent probes for STED nanoscopy and discusses the recent progress in the development of fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, for the STED nanoscopy. The strengths and the limitations of the fluorescent probes are analyzed in detail.

Indexed as

fluorescent nanoparticlefluorescent probefluorescent proteinorganic dyeSTEDsuper-resolution microscopy

Identifiers

PMID35009972
PMCPMC8746377

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.