ArticleInternational journal of molecular sciences2021
An Efficient Protein Evolution Workflow for the Improvement of Bacterial PET Hydrolyzing Enzymes.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Bio-Based Polymers with Potential Antimicrobial Activity from Vanillin Methacrylate via ARGET-ATRP.Polymers · 2026Article
- Rethinking plastic waste: innovations in enzymatic breakdown of oil-based polyesters and bioplastics.FEBS open bio · 2026Review
- Molecular dynamics simulations of temperature-dependent PET binding in PETase, ThermoPETase, and FAST-PETase.RSC advances · 2026Article
- Protein biochemistry and engineering drive the development of a carbonic anhydrase-based carbon dioxide sequestration strategy.The FEBS journal · 2025Article
- Enzyme Benchmarking with Polyethylene Furanoate Soluble Scaffolds for Directed Evolution of PEFases.ACS omega · 2024Article
- Measuring PETase enzyme kinetics by single-molecule microscopy.Biophysical journal · 2024Article
- Article
- Influence of Wobbling Tryptophan and Mutations on PET Degradation Explored by QM/MM Free Energy Calculations.Journal of chemical information and modeling · 2024Article
- Display of PETase on the Cell Surface of Escherichia coli Using the Anchor Protein PgsA.Applied biochemistry and biotechnology · 2024Article
- Biochemical Characterisation of Sis: A Distinct Thermophilic PETase with Enhanced NanoPET Substrate Hydrolysis and Thermal Stability.International journal of molecular sciences · 2024Article
- Exploring the pH dependence of an improved PETase.Biophysical journal · 2024Article
- Measuring PETase enzyme kinetics by single-molecule microscopy.bioRxiv : the preprint server for biology · 2024Article
- Recent advances in microbial and enzymatic engineering for the biodegradation of micro- and nanoplastics.RSC advances · 2024Review
- Improving plastic degrading enzymes via directed evolution.Protein engineering, design & selection : PEDS · 2024Review
- Mining thermophiles for biotechnologically relevant enzymes: evaluating the potential of European and Caucasian hot springs.Extremophiles : life under extreme conditions · 2023Review
- A PETase enzyme synthesised in the chloroplast of the microalga Chlamydomonas reinhardtii is active against post-consumer plastics.Scientific reports · 2023Article
- Microbial Enzyme Biotechnology to Reach Plastic Waste Circularity: Current Status, Problems and Perspectives.International journal of molecular sciences · 2023Review
- An Ultra-SensitiveApplied and environmental microbiology · 2023Article
- Trends inComputational and structural biotechnology journal · 2023Review
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Enzymatic degradation is a promising green approach to bioremediation and recycling of the polymer poly(ethylene terephthalate) (PET). In the past few years, several PET-hydrolysing enzymes (PHEs) have been discovered, and new variants have been evolved by protein engineering. Here, we report on a straightforward workflow employing semi-rational protein engineering combined to a high-throughput screening of variant libraries for their activity on PET nanoparticles. Using this approach, starting from the double variant W159H/S238F of
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Registered trials
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