Evidence map›Paper›PMID 35008562›Full record

ArticleInternational journal of molecular sciences2021

Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.

Davide Facchetti, Ute Hempel, Laurine Martocq, Alan M Smith, Andrey Koptyug, Roman A Surmenev, Maria A Surmeneva, Timothy E L Douglas

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Biopolymers in Drug and Gene Delivery Systems.International journal of molecular sciences · 2023
    Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 4 countries.

Davide FacchettiEngineering Department, Lancaster University, Lancaster LA1 4YW, UK.ORCID 0000-0001-8290-289X
Ute HempelInstitute of Physiological Chemistry, Technische Universität Dresden, 01307 Dresden, Germany.
Laurine MartocqEngineering Department, Lancaster University, Lancaster LA1 4YW, UK.ORCID 0000-0002-7579-965X
Alan M SmithDepartment of Pharmacy, School of Applied Sciences, University of Huddersfield, Huddersfield HD1 3DR, UK.ORCID 0000-0003-2791-3208
Andrey KoptyugDepartment of Quality Technology, Mechanical Engineering & Mathematics, Mid Sweden University, 831 25 Ostersund, Sweden.ORCID 0000-0003-2964-9500
Roman A SurmenevPhysical Materials Science and Composite Materials Centre, Research School of Chemistry & Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.ORCID 0000-0002-8061-3047
Maria A SurmenevaPhysical Materials Science and Composite Materials Centre, Research School of Chemistry & Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.
Timothy E L DouglasEngineering Department, Lancaster University, Lancaster LA1 4YW, UK.
Lancaster University · GBNational Research Tomsk State University · RUImperial College London · GBMid Sweden University · SETechnische Universität Dresden · DEUniversity of Huddersfield · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Titanium alloy (Ti6Al4V) is one of the most prominent biomaterials for bone contact because of its ability to bear mechanical loading and resist corrosion. The success of Ti6Al4V implants depends on bone formation on the implant surface. Hence, implant coatings which promote adhesion, proliferation and differentiation of bone-forming cells are desirable. One coating strategy is by adsorption of biomacromolecules. In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells. WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC). Indeed, WPI fibrils were resistant to sterilization and were stable during storage. This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity. Finally, the coating increased the hydrophilicity of the material. The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments. Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.

Indexed as

AdultAlkaline PhosphataseAlloysBiocompatible MaterialsBone and BonesCell DifferentiationCell ProliferationCells, CulturedCoated Materials, BiocompatibleHeparinHumansHydrophobic and Hydrophilic InteractionsMaleMesenchymal Stem CellsOsteogenesisTitaniumAlkaline PhosphataseAlloysBiocompatible MaterialsCoated Materials, BiocompatibleHeparinTitaniumtitanium alloy (TiAl6V4)Whey Proteinsadditive manufacturingcoatingenrichedheparinosseointegrationosteoblast differentiationTi6Al4VtinzaparinWPI fibrils

Identifiers

PMID35008562
PMCPMC8745389
OpenAlexW4200331684

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.