ArticleInternational journal of molecular sciences2021
Whole-Tissue Three-Dimensional Imaging of Rice at Single-Cell Resolution.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Research on Bamboo Shoot Bud Development: A Leap from Tissue Heterogeneity to Single-Cell Spatial Atlas.Plants (Basel, Switzerland) · 2026Review
- Florigen and cytokinin signaling antagonistically regulate FLOWERING LOCUS T-LIKE1 to drive a florigen relay that facilitates inflorescence development in rice.Science advances · 2025Article
- The Function of Florigen in the Vegetative-to-Reproductive Phase Transition in and around the Shoot Apical Meristem.Plant & cell physiology · 2024Review
- 3D multiple immunoimaging using whole male organs in rice.Scientific reports · 2022Article
- Current Advances and Future Prospects for Molecular Research for Agronomically Important Traits in Rice.International journal of molecular sciences · 2022Article
- Auxin Distribution in Lateral Root Primordium Development Affects the Size and Lateral Root Diameter of Rice.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The three-dimensional (3D) arrangement of cells in tissues provides an anatomical basis for analyzing physiological and biochemical aspects of plant and animal cellular development and function. In this study, we established a protocol for tissue clearing and 3D imaging in rice. Our protocol is based on three improvements: clearing with iTOMEI (clearing solution suitable for plants), developing microscopic conditions in which the Z step is optimized for 3D reconstruction, and optimizing cell-wall staining. Our protocol successfully 3D imaged rice shoot apical meristems, florets, and root apical meristems at cellular resolution throughout whole tissues. Using fluorescent reporters of auxin signaling in rice root tips, we also revealed the 3D distribution of auxin signaling events that are activated in the columella, quiescent center, and multiple rows of cells in the stele of the root apical meristem. Examination of cells with higher levels of auxin signaling revealed that only the central row of cells was connected to the quiescent center. Our method provides opportunities to observe the 3D arrangement of cells in rice tissues.
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Registered trials
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