ArticleMolecular ecology resources2022
Genotyping-in-thousands by sequencing (GT-seq) of noninvasive faecal and degraded samples: A new panel to enable ongoing monitoring of Canadian polar bear populations.
Article in Molecular ecology resources, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 37 citations in OpenAlex.
- A Genomic Tool to Tackle Cryptic Diversity Demonstrates the Potential for Off-Target Use of GT-Seq Panels.Molecular ecology resources · 2026Article
- Development of a SNP Amplicon Panel for Invasive Deer Faecal Pellets and the Effects of Environmental Exposure on Genotyping Success.Ecology and evolution · 2026Article
- Direct microhaplotype genotyping for GT-seq (Genotyping-in-Thousands by Sequencing) using a diploid abundance model.PLoS computational biology · 2026Article
- Approaches to Thoroughbred Horse Identification in Diverse Sample Types Using Multiplexed SNP-PCR Sequencing Panels.Animal genetics · 2026Article
- Leveraging Next-Generation Tools for Genetic Assessment and Demographic Monitoring in Threatened and Elusive Humboldt Martens.Evolutionary applications · 2026Article
- Genome-Wide SNP Diversity in Natural and Cultivated Populations Informs Restoration Ecology With Three Calamagrostis Species From the Northwest Territories of Canada.Molecular ecology · 2026Article
- A Practical Framework for GT-Seq Panel Optimization.Molecular ecology resources · 2026Article
- GT-Seq Panel Development for Species Identification and Parentage Analysis of Closely Related Hybridising Scaphirhynchus Sturgeons.Molecular ecology resources · 2026Article
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- Development of High-Throughput Genomic Resources to Inform White-Tailed Deer Population and Disease Management.Molecular ecology resources · 2026Article
- Age Is Just a Number: No Impacts of Scat Ageing on Single Nucleotide Polymorphism Genotyping Using a Target Capture Approach.Ecology and evolution · 2025Article
- Genetic Variation and Metapopulation Structure Inform Recovery Goals in a Threatened Species.Genes · 2025Article
- Development of a Genotyping-in-Thousands by Sequencing (GT-Seq) Panel for Identifying Individuals and Estimating Relatedness Among Alaska Black Bears (Ecology and evolution · 2025Article
- Development of a Noninvasive Genotyping-In-Thousands (GTseq) Panel for Long-Term Conservation of Western Great Lakes Gray Wolves (Ecology and evolution · 2025Article
- MhGeneS: An Analytical Pipeline to Allow for Robust Microhaplotype Genotyping.Molecular ecology resources · 2025Article
- Identification and application of a candidate gene AhAftr1 for aflatoxin production resistance in peanut seed (Arachis hypogaea L.).Journal of advanced research · 2024Article
- FecalSeq enrichment with RAD Sequencing from non-invasive environmental samples holds promise for genetic monitoring of an imperiled lagomorph.Scientific reports · 2024Article
- Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples.Scientific reports · 2024Article
- The geographic distribution, and the biotic and abiotic predictors of select zoonotic pathogen detections in Canadian polar bears.Scientific reports · 2024Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Genetic monitoring using noninvasive samples provides a complement or alternative to traditional population monitoring methods. However, next-generation sequencing approaches to monitoring typically require high quality DNA and the use of noninvasive samples (e.g., scat) is often challenged by poor DNA quality and contamination by nontarget species. One promising solution is a highly multiplexed sequencing approach called genotyping-in-thousands by sequencing (GT-seq), which can enable cost-efficient genomics-based monitoring for populations based on noninvasively collected samples. Here, we develop and validate a GT-seq panel of 324 single nucleotide polymorphisms (SNPs) optimized for genotyping of polar bears based on DNA from noninvasively collected faecal samples. We demonstrate (1) successful GT-seq genotyping of DNA from a range of sample sources, including successful genotyping (>50% loci) of 62.9% of noninvasively collected faecal samples determined to contain polar bear DNA; and (2) that we can reliably differentiate individuals, ascertain sex, assess relatedness, and resolve population structure of Canadian polar bear subpopulations based on a GT-seq panel of 324 SNPs. Our GT-seq data reveal spatial-genetic patterns similar to previous polar bear studies but at lesser cost per sample and through use of noninvasively collected samples, indicating the potential of this approach for population monitoring. This GT-seq panel provides the foundation for a noninvasive toolkit for polar bear monitoring and can contribute to community-based programmes - a framework which may serve as a model for wildlife conservation and management for species worldwide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.