ArticleDevelopment (Cambridge, England)2022
Zebrafish vascular quantification: a tool for quantification of three-dimensional zebrafish cerebrovascular architecture by automated image analysis.
Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A Framework for Selecting Zebrafish Models of Metabolic Diseases: Modeling Strategies, Phenotypic Validation, Mechanistic Investigation, and Efficacy Evaluation.Biomedicines · 2026Review
- Quantitative Preclinical Imaging as a Metrological Framework: Reproducibility, Validation, and Translational Maturity.Journal of imaging · 2026Review
- Automated analysis of zebrafish vascular networks using the VISTA-Z pipeline.Scientific reports · 2026Article
- Emerging Light-Sheet Fluorescence Microscopy for Cardiac Vascular Imaging in Zebrafish.Current treatment options in cardiovascular medicine · 2026Review
- Zebrafish model for studying vascular development and vascular-related disease.Frontiers in cell and developmental biology · 2026Review
- Notch4 is essential for the maintenance of vascular homeostasis in the young adult pituitary posterior lobes.Scientific reports · 2025Article
- IL-1β disrupts the initiation of blood-brain barrier development by inhibiting endothelial Wnt/β-catenin signaling.iScience · 2024Article
- Three-dimensional identification of microvascular pathology and neurovascular inflammation in severe white matter hyperintensity: a case report.Scientific reports · 2024Article
- Development and repair of blood vessels in the zebrafish spinal cord.Open biology · 2023Article
- Quantitative characterization of zebrafish development based on multiple classifications using Mueller matrix OCT.Biomedical optics express · 2023Article
- Therapeutic targeting of vascular malformation in a zebrafish model of hereditary haemorrhagic telangiectasia.Disease models & mechanisms · 2023Article
- GliaMorph: a modular image analysis toolkit to quantify Müller glial cell morphology.Development (Cambridge, England) · 2023Article
- Three-dimensional cerebral vasculature topological parameter extraction of transgenic zebrafish embryos with a filling-enhancement deep learning network.Biomedical optics express · 2023Article
- Q-VAT: Quantitative Vascular Analysis Tool.Frontiers in cardiovascular medicine · 2023Article
Corrections and comments
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Authors and funding
7 authors.
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Abstract
Zebrafish transgenic lines and light sheet fluorescence microscopy allow in-depth insights into three-dimensional vascular development in vivo. However, quantification of the zebrafish cerebral vasculature in 3D remains highly challenging. Here, we describe and test an image analysis workflow for 3D quantification of the total or regional zebrafish brain vasculature, called zebrafish vasculature quantification (ZVQ). It provides the first landmark- or object-based vascular inter-sample registration of the zebrafish cerebral vasculature, producing population average maps allowing rapid assessment of intra- and inter-group vascular anatomy. ZVQ also extracts a range of quantitative vascular parameters from a user-specified region of interest, including volume, surface area, density, branching points, length, radius and complexity. Application of ZVQ to 13 experimental conditions, including embryonic development, pharmacological manipulations and morpholino-induced gene knockdown, shows that ZVQ is robust, allows extraction of biologically relevant information and quantification of vascular alteration, and can provide novel insights into vascular biology. To allow dissemination, the code for quantification, a graphical user interface and workflow documentation are provided. Together, ZVQ provides the first open-source quantitative approach to assess the 3D cerebrovascular architecture in zebrafish.
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