Evidence map›Paper›PMID 35005700›Full record

ArticleFrontiers in genome editing2021

A Versatile and Efficient Plant Protoplast Platform for Genome Editing by Cas9 RNPs.

Wenzhi Jiang, Jenifer Bush, Jen Sheen

Open access · goldAbstract read
In one paragraph

Article in Frontiers in genome editing, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 28 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Tiny but powerful: protoplast isolation inFrontiers in plant science · 2026
    Article
  5. Article
  6. Article
  7. CRISPR-Cas Gene Editing Technology in Potato.International journal of molecular sciences · 2025
    Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Review
  15. Review
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Wenzhi JiangDepartment of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, and Department of Genetics, Harvard Medical School, Boston, MA, United States.
Jenifer BushDepartment of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, and Department of Genetics, Harvard Medical School, Boston, MA, United States.
Jen SheenDepartment of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, and Department of Genetics, Harvard Medical School, Boston, MA, United States.
Massachusetts General Hospital · USHarvard University · US

Funding

Plant Nutrient-Growth Signaling NetworkR01GM129093 · NIGMS · MASSACHUSETTS GENERAL HOSPITAL · PI SHEEN, JEN · 2018 to 2025
$2.8M
NIGMS NIH HHS R01 GM129093
6 · The paper itself

Abstract

The ultimate goal of technology development in genome editing is to enable precisely targeted genomic changes in any cells or organisms. Here we describe protoplast systems for precise and efficient DNA sequence changes with preassembled Cas9 ribonucleoprotein (RNP) complexes in

Indexed as

Arabidopsis thalianaCas9 RNPHDRNHEJNicotiana benthamianaplant protoplastsprime editor RNPssODN donor

Identifiers

PMID35005700
PMCPMC8729822
OpenAlexW4200268529

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.