Evidence map›Paper›PMID 35005639›Full record

ArticleSTAR protocols2022

Protocol for

Shupeng Dong, Xiaochun Xie, Yujie Chen, Gang Long, Qiming Liang, Zhen Zhao, Jianxiong Zeng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Shupeng DongResearch Center of Translational Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200062, China.
Xiaochun XieDepartment of Physiology and Neuroscience, University of Southern California, Los Angeles, CA 90033, USA.
Yujie ChenKey Laboratory of Molecular Virology and Immunology, Institute Pasteur of Shanghai, Shanghai Institute for Biological Science, Chinese Academy of Sciences, Shanghai, China.
Gang LongKey Laboratory of Molecular Virology and Immunology, Institute Pasteur of Shanghai, Shanghai Institute for Biological Science, Chinese Academy of Sciences, Shanghai, China.
Qiming LiangResearch Center of Translational Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200062, China.
Zhen ZhaoDepartment of Physiology and Neuroscience, University of Southern California, Los Angeles, CA 90033, USA.
Jianxiong ZengDepartment of Physiology and Neuroscience, University of Southern California, Los Angeles, CA 90033, USA.
University of Southern California · USInstitut Pasteur of Shanghai · CNShanghai Jiao Tong University · CN

Funding

Zika Virus Capsid Protein Mediated Blockage of host microRNA machineryR01NS110687 · NINDS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI ZHAO, ZHEN · 2019 to 2023
$2.2M
NINDS NIH HHS R01 NS110687
6 · The paper itself

Abstract

Zika virus (ZIKV) is linked to congenital defects including microcephaly. An infection model that can recapitulate most microcephaly-related phenotypes is crucial for understanding ZIKV pathogenesis. Here, we present a protocol to generate ZIKV from an infectious clone through a reverse genetic system and subsequently perform embryonic brain infection with the rescued ZIKV in pregnant mice. We optimized several aspects of the procedures including virus rescue and

Indexed as

MicrocephalyNervous System MalformationsZika VirusZika Virus InfectionAnimalsBody Weights and MeasuresFemaleMicePregnancyMicrobiologyMolecular BiologyNeuroscienceStem Cells

Identifiers

PMID35005639
PMCPMC8715324
OpenAlexW4200366100

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.