Evidence map›Paper›PMID 35005635›Full record

ArticleSTAR protocols2022

High-throughput screening for agonists of ROS production in live human vascular endothelial cells.

Tomoya Sasahara, Minako Hoshi

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Tomoya SasaharaDepartment for Brain and Neurodegenerative Disease Research, Institute of Biomedical Research and Innovation, Foundation for Biomedical Research and Innovation at Kobe, CLIK 6F 6-3-7 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan.
Minako HoshiDepartment for Brain and Neurodegenerative Disease Research, Institute of Biomedical Research and Innovation, Foundation for Biomedical Research and Innovation at Kobe, CLIK 6F 6-3-7 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan.
Foundation for Biomedical Research and Innovation · JPKyoto University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reactive oxygen species (ROS) are important physiological molecules, and identifying agonists for ROS production can yield useful tools for future research. Here we present an optimized protocol for high-throughput screening for agonists that induce ROS production. We describe the use of a fluorescent probe in human vascular endothelial cells, which can establish whether ROS production occurs in mitochondria or in the plasma membrane of live cells. For complete details on the use and execution of this profile, please refer to Sasahara et al. (2021).

Indexed as

Endothelial CellsHigh-Throughput Screening AssaysFluorescent DyesHumansMitochondriaReactive Oxygen SpeciesFluorescent DyesReactive Oxygen SpeciesCell-based AssaysCell BiologyHigh Throughput ScreeningMicroscopyMolecular/Chemical Probes

Identifiers

PMID35005635
PMCPMC8715207
OpenAlexW4200550155

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.