ArticleMethodsX2021
A practical method for storage, preservation and transportation of anuran urine samples using filter paper for hormone analysis.
Article in MethodsX, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Investigating Coriander Leaf Phenolics With HPLC-UV and Their Role in Modulating Nitrogen Metabolism.Food science & nutrition · 2025Article
- Use of ash filtrate as an alternative to chemical disinfectant and its antimicrobial efficacy.Journal of advanced veterinary and animal research · 2024Article
- Quantification of quercetin from red onion (Food science & nutrition · 2024Article
- Measures of Adrenal and Gonadal Hormones in Relation to Biological and Management Factors among Captive Red Pandas in Indian Zoos.Animals : an open access journal from MDPI · 2023Article
- Development of an enzyme immunoassay to measure urinary and faecal 5α-androst-16-en-3-one in pigs.MethodsX · 2023Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
Anurans (frogs and toads) expelled urine when handled and it could provide insights into their physiological status. However, storage, preservation and transportation are often challenging. The study aimed to standardize and validate a field method for short-term storage and preserve of anuran urine samples using Whatman filter papers. To examine the efficacy of storage conditions and type of papers, urinary based enzyme immunoassays were used to measure progesterone and testosterone hormone metabolites. High-Performance Liquid Chromatography was performed and revealed immunoreactive progesterone and testosterone metabolites in the urine samples. Urinary hormone metabolites concentration stored in filter paper at room temperature and control samples stored in -20°C for the same period were similar. Whatman grade 50 was found to be more suitable for storage of hormones than grade 3 paper for the experiments performed.•A cheap and simple storage method for storage of anuran urine in field conditions using filter papers.•Anuran urine could be preserved and transported under ambient conditions without significant changes and loss of hormones.•This method would facilitate the endocrine monitoring of anurans in remote areas where limited logistics are available.
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Registered trials
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