Magnetic bead purification of enveloped alphavirus and flavivirus.

Bianca Galasso, Shreya Sharma, Barbara Knollmann-Ritschel, Anuj Sharma

Abstract read

In one paragraph

Article in MethodsX, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Exploring Mosquito Excreta as an Alternative Sample Type for Improving Arbovirus Surveillance in Australia.Pathogens (Basel, Switzerland) · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Bianca GalassoDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.

Shreya SharmaDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.

Barbara Knollmann-RitschelDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.

Anuj SharmaDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Virus production is an essential part of virology. The quality of virus preparation, in terms of purification and yield, can affect the outcome of experiments. Purified virus is critical for assays, as contamination from cell culture supernatants may affect results. Purified virus is also needed for vaccine preparations. The traditional process of gradient purification of virus is time consuming, with multiple steps spanning over days, and produces significant amounts of biohazard waste. Developing a more efficient alternative that provides a purified virus product is highly desirable. In this study, we performed magnetic bead purification of Zika virus (ZIKV) and Mayaro virus (MAYV) with the Mag4C LV kit from OZ Biosciences. Currently, this kit is only indicated for use with lentiviruses and retroviruses. We found that we were able to successfully concentrate and purify both ZIKV and MAYV using the kit. Therefore, we concluded that the Mag4C LV kit provides a quick and simple alternative to traditional virus purification methods.•Our protocol is customized by using an alphavirus (MAYV) and flavivirus (ZIKV). This method has not been previously used for these viruses.

Indexed as

AlphavirusFlavivirusMagnetic bead purificationMayaro virusVaccinesVirologyVirus purificationVirus purityVirus titerZika virus

Identifiers

PMID35004196

DOI10.1016/j.mex.2021.101549

PMCPMC8720801

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.