ArticleBiomedical optics express2021
Phototoxicity induced in living HeLa cells by focused femtosecond laser pulses: a data-driven approach.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A versatile platform for two-photon neuronal population voltage imaging across cortical depths.Nature methods · 2026Article
- Upconversion Photoluminescence to Monitor Local Heat Release During Femtosecond Direct Laser Writing of Bioinks In Situ.Small methods · 2026Article
- Unveiling Exciton-Plasmon Polariton Coupling Regions via Polarization-Enhanced Optical Nanoscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Dynamic imaging of myelin pathology in physiologically preserved human brain tissue using third harmonic generation microscopy.PloS one · 2025Article
- Spatially-Resolved Organoid Transfection by Porous Silicon-Mediated Optoporation.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- More than double the fun with two-photon excitation microscopy.Communications biology · 2024Review
- Development of label-free light-controlled gene expression technologies using mid-IR and terahertz light.Frontiers in bioengineering and biotechnology · 2024Review
- Bond-selective fluorescence imaging with single-molecule sensitivity.Nature photonics · 2023Article
- Label-Free Optical Metabolic Imaging in Cells and Tissues.Annual review of biomedical engineering · 2023Review
- Video-rate Raman-based metabolic imaging by Airy light-sheet illumination and photon-sparse detection.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Recent Advances in Raman Spectral Imaging in Cell Diagnosis and Gene Expression Prediction.Genes · 2022Review
- Low-intensity illumination for lensless digital holographic microscopy with minimized sample interaction.Biomedical optics express · 2022Article
- Transient absorption spectroscopy and imaging of redox in muscle mitochondria.Biomedical optics express · 2022Article
- Phototoxicity induced in living HeLa cells by focused femtosecond laser pulses: a data-driven approach.Biomedical optics express · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
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Abstract
Nonlinear optical microscopy is a powerful label-free imaging technology, providing biochemical and structural information in living cells and tissues. A possible drawback is photodamage induced by high-power ultrashort laser pulses. Here we present an experimental study on thousands of HeLa cells, to characterize the damage induced by focused femtosecond near-infrared laser pulses as a function of laser power, scanning speed and exposure time, in both wide-field and point-scanning illumination configurations. Our data-driven approach offers an interpretation of the underlying damage mechanisms and provides a predictive model that estimates its probability and extension and a safety limit for the working conditions in nonlinear optical microscopy. In particular, we demonstrate that cells can withstand high temperatures for a short amount of time, while they die if exposed for longer times to mild temperatures. It is thus better to illuminate the samples with high irradiances: thanks to the nonlinear imaging mechanism, much stronger signals will be generated, enabling fast imaging and thus avoiding sample photodamage.
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