ArticleBiomedical optics express2021
Quantification of cytochrome c oxidase and tissue oxygenation using CW-NIRS in a mouse cerebral cortex.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
7 citing papers in PubMed, 1 synthesis or guideline pooled it, 12 citations in OpenAlex.
- Advancements in broadband near-infrared spectroscopy instrumentation for the assessment ofJournal of biomedical optics · 2025Pooled it
- Prediction of treatment responsiveness to home-based transcranial photobiomodulation (tPBM) intervention for cognitive decline using fNIRS concurrently recorded during tPBM.Frontiers in aging neuroscience · 2026Article
- Quantification of oxidized and reduced cytochrome-c-oxidase by combining discrete-wavelength time-resolved and broadband continuous-wave near-infrared spectroscopy.Biomedical optics express · 2025Article
- The relationship between cytochrome c oxidase, CBF and CMROJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023Article
- Watching the human retina breath in real time and the slowing of mitochondrial respiration with age.Scientific reports · 2023Article
- The Effect of Hypercapnia on Cortical Metabolic Rate and Mitochondrial Redox Status.Advances in experimental medicine and biology · 2023Article
- Multimodal NIRS-MRI reveals cortical hypoperfusion, hypoxia, and mitochondrial changes in a mouse model of autoimmune inflammatory diseases.Imaging neuroscience (Cambridge, Mass.)Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We provide a protocol for measuring the absolute concentration of the oxidized and reduced state of cytochrome c oxidase (CCO) in the cerebral cortex of mice, using broadband continuous-wave NIRS. The algorithm (NIR-AQUA) allows for absolute quantification of CCO and deoxyhemoglobin. Combined with an anoxia pulse, this also allows for quantification of total hemoglobin, and tissue oxygen saturation. CCO in the cortex was 4.9 ± 0.1 μM (mean ± SD, n=6). In normoxia, 84% of CCO was oxidized. We include hypoxia and cyanide validation studies to show CCO can be quantified independently to hemoglobin. This can be applied to study oxidative metabolism in the many rodent models of neurological disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.